In vitro investigations of biological activities of Thymus willdenowii and Thymus atlanticus polyphenol-rich extracts

Elbouny Hamza, Ouahzizi Brahi̇m, S. Khalid, Alem Chakib
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引用次数: 2

Abstract

Thyme species produce a wide variety of phenolic compounds including tannins, phenolic acids, and flavonoids. Thymus atlanticus (T. atlanticus) and Thymus willdenowii (T. willdenowii) are important thyme species in the southeast of Morocco, with numerous biological properties. The polyphenolic extracts of these two thyme species were obtained using ethanol through Soxhlet apparatus. Antioxidant (DPPH, FRAP, and TAC methods), antihemolytic (2,2'-Azobis(2-amidinopropane) dihydrochloride (AAPH) induced hemolysis test), hypolipidemic (3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase activity inhibition test), and anti-inflammatory (protein denaturation inhibition) effects of extracts were carried out using in vitro methods. The results showed that the polyphenolic extracts of these two species revealed important amounts of phenolic compounds. The contents of flavonoids were significant in the two species, while the contents of tannins and anthocyanin were very low. T. atlanticus showed an important antioxidant activity and a considerable antihemolytic effect in AAPH-induced hemolysis test (IC50 = 0.29 mg/mL), while T. willdenowii showed an important anti-inflammatory activity in heat-induced protein denaturation test (IC50 = 1.61 mg/mL). Moreover, both extracts at a dose of 20 µg/mL showed an important in vitro hypolipidemic activity by inhibiting HMG-CoA reductase activity (T. willdenowii: 51.16 %; T. atlanticus: 62.83 %). In conclusion, T. willdenowii and T. atlanticus extracts have considerable antioxidant, antihemolytic, hypolipidemic, and anti-inflammatory effects. The richness of these species in polyphenols gives them a large specter of biological properties, making them a valuable source of natural bioactive compounds that could prevent or treat various diseases.
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willdenowii和atlanticus富含多酚提取物的体外生物活性研究
百里香产生多种酚类化合物,包括单宁、酚酸和黄酮类化合物。atlanticus和willdenowii是摩洛哥东南部的重要百里香物种,具有许多生物学特性。这两种百里香的多酚提取物是用乙醇通过索氏装置获得的。使用体外方法进行提取物的抗氧化(DPPH、FRAP和TAC方法)、抗溶血(2,2'-偶氮双(2-氨基丙烷)二盐酸盐(AAPH)诱导的溶血试验)、降血脂(3-羟基-3-甲基戊二酰辅酶A(HMG-CoA)还原酶活性抑制试验)和抗炎(蛋白质变性抑制)作用。结果表明,这两种植物的多酚提取物中含有大量的酚类化合物。两种植物中黄酮类化合物含量显著,而单宁和花青素含量极低。atlanticus在AAPH诱导的溶血试验中显示出重要的抗氧化活性和相当大的抗溶血作用(IC50=0.29 mg/mL),而willdenowii在热诱导的蛋白质变性试验中表现出重要的抗炎活性(IC50=1.61 mg/mL)。此外,两种提取物在20µg/mL的剂量下均通过抑制HMG-CoA还原酶活性显示出重要的体外降血脂活性(T.willdenwii:51.16%;T.atlanticus:62.83%)。总之,willdenowii和T.atlanticus提取物具有相当大的抗氧化、解热、降血脂和抗炎作用。这些物种富含多酚,这使它们具有很大的生物特性,成为预防或治疗各种疾病的天然生物活性化合物的宝贵来源。
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来源期刊
International Journal of Secondary Metabolite
International Journal of Secondary Metabolite Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
1.40
自引率
0.00%
发文量
28
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