Inactivation rates of African swine fever virus by compound disinfectants

IF 3.5 2区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Annals of Agricultural Science Pub Date : 2022-12-01 DOI:10.1016/j.aoas.2022.11.002
Van Phan Le , Tapanut Songkasupa , Prakit Boonpornprasert , Thi Lan Nguyen , Suphachai Nuanualsuwan
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Abstract

African swine fever is an important viral disease of domestic pigs and wild boars. Inactivation of the African swine fever virus (ASFV) contaminating equipment and premises is an effective biosecurity risk management measure to prevent the introduction of ASFV through contaminated fomites and the environment. Even though some chemical agents' virucidal activity against ASFV was previously demonstrated, compound disinfectants are still limited. For the fact that the ASFV titer in some excretions from pigs was far >4 log. Even after the application of disinfectants as instructed by the manufacturers, the excessive ASFV load in the excretions from pigs could contaminate fomites or the environment. To solve this dilemma, the concept of contact time of disinfectant for 1–log reduction was introduced, as the decimal reduction time or D value. Therefore, the objective of this study was to assess the inactivation rate of four compound disinfectants against ASFV as the D value. Four compound disinfectants (iodine and phenol, quaternary ammonium and alcohol, and two quaternary ammonium and glutaraldehyde compounds) currently used in the veterinary field were evaluated in a suspension test at 25 °C in the absence of interfering substances. Primary porcine alveolar macrophages were used to determine the titer of ASFV genotype II (strain VNUA/HY-ASF1/Vietnam/2019). The virucidal activity of four compound disinfectants achieved 4–log ASFV reduction. The mean D0.5%, D0.25%, and D0.125% values of all disinfectants inactivating ASFV were 1.62–3.60, 2.60–3.8, and 2.89–3.90 min, respectively. The D value of disinfectants allows users to manipulate the appropriate concentration and/or contact time of the disinfectant, depending on the magnitude of ASFV contamination, to completely inactivate the ASFV titer on the fomites to minimise a potential biosecurity risk.

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复方消毒剂对非洲猪瘟病毒灭活率的影响
非洲猪瘟是家猪和野猪的一种重要病毒性疾病。对污染设备和场所的非洲猪瘟病毒(ASFV)进行灭活是一项有效的生物安全风险管理措施,可防止通过污染的污染物和环境传入非洲猪瘟病毒。尽管一些化学制剂对非洲猪瘟病毒的杀病毒活性先前已得到证实,但复合消毒剂仍然有限。部分猪排泄物中ASFV滴度高达4倍。即使按照生产商的指示使用了消毒剂,猪排泄物中过量的非洲猪瘟病毒仍可能污染污染物或环境。为了解决这一困境,引入了1对数还原消毒接触时间的概念,即十进制还原时间或D值。因此,本研究的目的是评估四种复方消毒剂对非洲猪瘟病毒的失活率作为D值。在25°C无干扰物质条件下,对目前兽医领域使用的四种复合消毒剂(碘与苯酚、季铵与醇、两种季铵与戊二醛化合物)进行了悬浮液试验。采用原代猪肺泡巨噬细胞检测ASFV基因II型(VNUA/HY-ASF1/Vietnam/2019)的滴度。4种复方消毒剂的杀病毒活性均达到4倍的降低。各消毒剂灭活ASFV的平均D0.5%、D0.25%和D0.125%分别为1.62 ~ 3.60、2.60 ~ 3.8和2.89 ~ 3.90 min。消毒剂的D值允许使用者根据非洲猪瘟病毒污染的程度操纵消毒剂的适当浓度和/或接触时间,以完全灭活污染物上的非洲猪瘟病毒滴度,以尽量减少潜在的生物安全风险。
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来源期刊
Annals of Agricultural Science
Annals of Agricultural Science AGRICULTURE, MULTIDISCIPLINARY-
CiteScore
12.60
自引率
0.00%
发文量
18
审稿时长
33 days
期刊介绍: Annals of Agricultural Sciences (AOAS) is the official journal of Faculty of Agriculture, Ain Shams University. AOAS is an open access peer-reviewed journal publishing original research articles and review articles on experimental and modelling research at laboratory, field, farm, landscape, and industrial levels. AOAS aims to maximize the quality of the agricultural sector across the globe with emphasis on the Arabian countries by focusing on publishing the high-quality applicable researches, in addition to the new methods and frontiers leading to maximizing the quality and quantity of both plant and animal yield and final products.
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