Effects of divided injection on the survival of fat graft in nude mice

Abstract

Objective To explore the effect of divided injection of fat grafting and to provide a new sight for the strategy of clinical particle fat transplantation. Methods Adipose tissue was aspirated from the healthy female abdomen by liposuction. In the control group, 0.5 ml of adipose tissue was subcutaneously injected into the nude mice. The experimental group was injected with 0.25 ml first, followed by 0.25 ml injection of adipose tissue on the 7th, 14th and 30th days. To assess graft retention rate and effectiveness we measured the wet weight and observed the pathological sections with HE or perilipin immunofluorescence. Results The wet weight of the tissue between the experimental group and the control group had no statistical difference, the experimental group had less necrosis and empty tissue than the control group. The proportion of perilipin positive staining tissue in 7 day group had statistical difference from that of the control group. Conclusions The strategy of pre-injection of part of the adipose tissue and then supplement of the residual tissue after 7 days may increase the proportion of active adipose tissue in the graft. Key words: Adipose tissue; Cell transplantation; Animal experiment; Fat granule; Divided injection