Effect of hydrogen sulfide on the proliferation of rabbit vascular smooth muscle cell under high static pressure

中华普通外科杂志 Pub Date : 2020-03-25 DOI:10.3760/CMA.J.CN113855-20190520-00269

T. He, Jie Wu, Changsheng Xu, Pian-Pian Chen, Fanggang Cai

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Abstract

Objective To observe the effect of exogenous hydrogen sulfide(H2S) on the proliferation of rabbit vascular smooth muscle cell(VSMC) under high static pressure. Methods Rabbit thoracic aorta VSMC were isolated and cultured under high static pressure(100mmHg) and divided into control group [cultured with 0.2%fetal bovine serum(FBS) and no NaHS]、10%FBS group(10%FBS and no NaHS) and NaHS group(10%FBS and 50mmol/L NaHS). VSMC proliferation was analyzed with BUDR. CSE, Calmodulin(p-CaM)and CyclinD1 protein levels of VSMC were measured by Western blotting. Results Compared with the 10%FBS group, NaHS inhibit the proliferation of rabbit VSMC significantly (0.50±0.03 vs. 0.26±0.03, P<0.05). Compared with control group, CSE protein in the 10%FBS group decreased significantly(1.21±0.10 vs. 0.33±0.04, P<0.05) and p-CaM and CyclinD1 protein increased significantly(0.23±0.04 vs. 0.86±0.04 and 0.22±0.03 vs. 1.19±0.06, P<0.05). Compared with the 10%FBS group, CSE protein in NaHS group increased significantly(0.33±0.04 vs. 1.11±0.11, P<0.05), and the expression of p-CaM and CyclinD1 protein decreased significantly(0.86±0.04 vs. 0.26±0.05 and 1.19±0.06 vs. 0.51±0.03, P<0.05). Conclusion Exogenous hydrogen sulfide inhibits the proliferation of VSMC under high static pressure by the CSE/H2S pathway which related to the reduction of the expression of p-CaM and CyclinD1. Key words: Hydrogen sulfide; Proliferation; Calmodulin; CyclinD1

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高压下硫化氢对兔血管平滑肌细胞增殖的影响

目的观察高静压条件下外源性硫化氢（H2S）对兔血管平滑肌细胞（VSMC）增殖的影响。方法分离培养兔胸主动脉VSMC，分为对照组（0.2%胎牛血清，不含NaHS）、10%FBS组（10%FBS，不含NaHS）和NaHS组（10%FB，50mmol/L NaHS）。用BUDR分析VSMC增殖。Western印迹法测定VSMC的CSE、钙调蛋白（p-CaM）和CyclinD1蛋白水平。结果与10%FBS组相比，NaHS对家兔VSMC增殖有显著抑制作用（0.50±0.03 vs.0.26±0.03，P<0.05），10%FBS组CSE蛋白显著下降（1.21±0.10 vs.0.33±0.04，P<0.05），P-CaM和CyclinD1蛋白显著增加（0.23±0.04 vs.0.86±0.04和0.22±0.03 vs.1.19±0.06，P<0.05），结论外源性硫化氢通过CSE/H2S途径抑制VSMC在高压下的增殖，这与p-CaM和CyclinD1的表达减少有关。关键词：硫化氢；扩散；钙调素；CyclinD1

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中华普通外科杂志

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