Role of Long Non-Coding RNA Zinc Finger E-Box Binding Homeobox 1 Antisense 1 in Regulating the microRNAs-200b/Wnt1 Axis Effects on Proliferation, Invasion, and Apoptosis of Gastric Carcinoma Cells

Nanoscience and Nanotechnology Letters Pub Date : 2020-04-01 DOI:10.1166/nnl.2020.3135

C. Mi, Li Ren, Dan Zhang, Shuixiang He

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Abstract

ZEB1-AS1 is an Long non-coding RNA (lncRNA) that is abnormally expressed in various tumor types and affects tumor etiology and progression. ZEB1-AS1 can affect the malignant progression of gastric cancer through the miRNA-200b/Wnt1 axis. However, its effect on proliferation, invasion, and apoptosis of gastric cancer is unclear. Using BGC-803 cells, we studied the effects of lncRNA ZEB1-AS1 and miRNA-200b on the proliferation, invasion, and apoptosis of gastric cancer. Using qRT-PCR, we found that the expression of ZEB1-AS1 was significantly increased and the expression of miRNA-200b was significantly decreased in BGC-803 cells. By CCK-8, Transwell, and flow cytometry assays, we also found that down-regulation of ZEB1-AS1 and upregulation of miRNA-200b inhibited the proliferation and invasion of BGC-803 cells and promoted apoptosis. Furthermore, upregulation of ZEB1-AS1 and miRNA-200b reversed these effects. LncRNA ZEB1-AS1 inhibits the proliferation and invasion of gastric cancer cells and induces apoptosis by regulating the miRNA-200b/Wnt1 molecular axis.

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长非编码RNA锌指E-盒结合同源框1反义1在调节微小RNAs-200b/Wnt1轴对胃癌细胞增殖、侵袭和凋亡影响中的作用

ZEB1-AS1是一种长链非编码RNA (lncRNA)，在各种肿瘤类型中异常表达，影响肿瘤的病因和进展。ZEB1-AS1可通过miRNA-200b/Wnt1轴影响胃癌的恶性进展。然而，其对胃癌的增殖、侵袭和凋亡的影响尚不清楚。我们利用BGC-803细胞，研究了lncRNA ZEB1-AS1和miRNA-200b对胃癌增殖、侵袭和凋亡的影响。通过qRT-PCR，我们发现在BGC-803细胞中，ZEB1-AS1的表达显著升高，miRNA-200b的表达显著降低。通过CCK-8、Transwell和流式细胞术检测，我们还发现下调ZEB1-AS1和上调miRNA-200b可抑制BGC-803细胞的增殖和侵袭，促进细胞凋亡。此外，ZEB1-AS1和miRNA-200b的上调逆转了这些作用。LncRNA ZEB1-AS1通过调控miRNA-200b/Wnt1分子轴抑制胃癌细胞的增殖和侵袭，诱导凋亡。

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Nanoscience and Nanotechnology Letters Physical, Chemical & Earth Sciences-MATERIALS SCIENCE, MULTIDISCIPLINARY

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2.6 months