Potassium amendment of inland saline groundwater: Insights into the physiological responses of silver pompano Trachinotus blochii (Lacepede, 1801) at different salinities

Pub Date : 2023-03-31 DOI:10.21077/ijf.2023.70.1.128657-10

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Abstract

A trial was conducted to evaluate the effect of graded levels of potassium (K+) amended inland saline groundwater (ISW) on serum ionic composition, antioxidant and metabolic enzymes [Na+/K+ATPase, Catalase, Superoxide dismutase (SOD), Glutathione peroxidase (GPx), Glutathione S-transferase (GST), Lactate dehydrogenase (LDH), aspartate aminotransferase (AST)] activities of silver pompano, Trachinotus blochii (Lacepede, 1801). The ISW was amended with 100% K+ at 5 ppt (ISW5), 10 ppt (ISW10) and 15 ppt (ISW15) and reconstituted seawater (RSW) with similar salinities (RSW5, RSW10 and RSW15) was employed as a reference medium. Experiment was conducted using completely randomised design. T. blochiijuveniles (1.5±0.5 g) were reared in 300 l capacity circular FRP tanks for 90 days. Fish were fed with commercial extruded floating feed. At the end of experimental period, serum ionic composition, enzymatic profiles and proximate composition were estimated. Results of the study showed that the serum ionic content (K+, Na+, Ca2+ and Mg2+) varied dose-dependently with salinity (p<0.05). Na+/K+ATPase, antioxidant enzymes (Catalase and SOD) and metabolic enzymes (GPx, GST, LDH and AST) showed higher activities at lower salinity (ISW5 and RSW5) compared to fish reared at 10 ppt and 15 ppt ISW. Similarly, proximate composition exhibited significantly (p<0.05) lower protein and lipid content at lower salinity (ISW5 and RSW5) signifying the stress condition at lower salinity. Physiological responses of T. blochii in terms of serum ionic composition, enzymatic profile and proximate composition were optimum at 10 and 15 ppt amended ISW, implying that rearing at 10-15 ppt with 100% K+ amendment of inland saline groundwater is appropriate. Keywords: Antioxidant activity, Inland saline groundwater, Metabolic enzymes, Potassium amendment, Proximate composition

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内陆含盐地下水的钾改良——对不同盐度条件下银蓬Trachinotus blochii（Lacepede，1801）生理反应的观察

本试验评价了不同水平的钾（K+）改良内陆盐水（ISW）对蓬帕诺银的血清离子组成、抗氧化和代谢酶[Na+/K+ATP酶、过氧化氢酶、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）、谷胱甘肽S-转移酶（GST）、乳酸脱氢酶（LDH）、天冬氨酸氨基转移酶（AST）]活性的影响，布氏Trachinotus blochii（Lacepede，1801）。用100%K+在5ppt（ISW5）、10ppt（IS-W10）和15ppt（ISW15）下修正ISW，并使用具有相似盐度的重组海水（RSW）（RSW5、RSW10和RSW15）作为参考介质。实验采用完全随机设计。T.blochiijuveniles（1.5±0.5 g）在300升容量的圆形玻璃钢罐中饲养90天。鱼类采用商业挤压漂浮饲料喂养。在实验结束时，对血清离子组成、酶谱和近似组成进行了估计。研究结果表明，血清离子含量（K+、Na+、Ca2+和Mg2+）随盐度呈剂量依赖性变化（p<0.05）。与10ppt和15ppt ISW饲养的鱼相比，Na+/K+ATP酶、抗氧化酶（过氧化氢酶和SOD）和代谢酶（GPx、GST、LDH和AST）在较低盐度（ISW5和RSW5）下表现出较高的活性。类似地，在较低盐度（ISW5和RSW5）下，接近成分表现出显著（p<0.05）较低的蛋白质和脂质含量，这表明在较低的盐度下存在应激条件。在10ppt和15ppt改良ISW条件下，布氏锥虫的血清离子组成、酶谱和近端组成的生理反应最为理想，这意味着在10-15ppt和100%K+改良内陆盐水条件下饲养是合适的。关键词：抗氧化活性；内陆盐水；代谢酶；钾改良剂；近似成分

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