Virulence and Reproduction of Entomopathogenic Nematodes Isolated from a Single Mexican Locality1

IF 0.7 4区 农林科学 Q4 ENTOMOLOGY Journal of Entomological Science Pub Date : 2023-09-07 DOI:10.18474/jes22-72
Diego Treviño-Cueto, Eduardo Hidalgo Mayorga, Sergei Subbotin, Melchor Cepeda Siller, S. Sanchez-Peña
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Abstract

Entomopathogenic nematodes (EPNs) are important biological control agents of insect pests. Strains or isolates obtained in specific regions and utilized in situ might contribute to the success of EPNs, since they are adapted to local abiotic conditions. We isolated and identified three isolates each of Heterorhabditis bacteriophora Poinar and Steinernema carpocapsae (Weiser) from soils at Saltillo, Coahuila State, Mexico. These six isolates were bioassayed against larvae of Tenebrio molitor L., using six concentrations of infective juveniles (IJs) per larva (0, 10, 25, 50, 100, 200). EPN-induced mortality of the isolates ranged from 15.2% to 100%. The designated M5 isolate of S. carpocapsae caused 100% mortality after 72 h at concentrations ≥25 IJs/larva with a median lethal concentration of 4.99 IJs/larva. Comparison of mortality levels induced at the same concentration of the six EPN isolates indicated that the Steinernema isolates, in general, induced higher mortality levels than the Heterorhabditis isolates. IJ production in T. molitor larvae also was greatest with the M5 isolate of S. carpocapsae following exposure of larvae to a concentration of 100 IJs/larva with a mean of 17,320 IJs/larva. Based on induced mortality and IJ production, the S. carpocapsae M5 isolate appears to be a viable candidate for further study and possible development for use in insect pest management programs. This study indicates that naturally coexisting local nematode isolates possess different attributes critical for their use as biocontrol agents.
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从墨西哥单一地区分离的昆虫病原线虫的毒力和繁殖1
昆虫病原线虫是重要的害虫生物防治剂。在特定地区获得并就地利用的菌株或分离物可能有助于epn的成功,因为它们适应当地的非生物条件。我们从墨西哥科阿韦拉州萨尔提略的土壤中分离鉴定了异habditis bacteriophora Poinar和steinerma carpocapsae (Weiser)各3株。采用6种不同浓度(0、10、25、50、100、200)的感染幼虫对黄粉虫幼虫进行生物检测。epn诱导的分离株死亡率为15.2% ~ 100%。指定M5分离物浓度≥25 IJs/幼虫72 h后死亡率为100%,中位致死浓度为4.99 IJs/幼虫。在相同浓度下对6株EPN分离株的死亡率进行比较,结果表明,Steinernema分离株的死亡率一般高于Heterorhabditis分离株。在暴露于浓度为100 IJs/幼虫时,稻角蛾M5分离株的IJ产量也最高,平均为17320 IJs/幼虫。基于诱导死亡率和IJ产量,S. carpocapsae M5分离物似乎是一个可行的候选物,可以进一步研究和开发用于害虫管理计划。这项研究表明,自然共存的本地线虫分离物具有不同的属性,这对它们作为生物防治剂的使用至关重要。
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来源期刊
CiteScore
1.20
自引率
11.10%
发文量
40
审稿时长
>12 weeks
期刊介绍: The Journal of Entomological Science (ISSN 0749-8004) is a peer-reviewed, scholarly journal that is published quarterly (January, April, July, and October) under the auspices of the Georgia Entomological Society in concert with Allen Press (Lawrence, Kansas). Manuscripts deemed acceptable for publication in the Journal report original research with insects and related arthropods or literature reviews offering foundations to innovative directions in entomological research
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