Uji Aktivitas Antioksidan dan Fenolik Total Fraksi Etil Asetat Daun Sembung (Blumea balsamifera (L.) DC)

Azimatur Rahmi, Tika Afriani, Linda Hevira, Wike Widiawati
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引用次数: 1

Abstract

The aim of this study is to investigate antioxidant activity and total phenolic content of ethyl acetate fraction of Sembung leaves. Sembung (Blumea balsamifera L.) is a plant that has the potential as an antioxidant because it contains secondary metabolites of alkaloids, steroids, flavonoids, saponins, and phenolic compounds. In this study, antioxidant activity was tested by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) for free radical scavenging activity assay. Evaluation of total phenolic content was tested by using the Folin-Ciocalteau method. The results showed that the ethyl acetate fraction of Sembung leaves possess showed medium antioxidant activity with IC50 valueof 221.821 compare with reference standard ascorbic acid with IC50 value 68.25 ppm. The ethyl acetate fraction of Sembung leaves has a total phenolic content of 161.101 mg GAE/g. This study provided that Blumea balsamifera leaves possess antioxidant.
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测试活性抗氧化剂和酚类总乙基组分Sembung Sheet Asset(Blumea balifera(L.)DC)
本研究的目的是研究Sembung叶乙酸乙酯部分的抗氧化活性和总酚含量。Sembung(Blumea ballifera L.)是一种具有抗氧化潜力的植物,因为它含有生物碱、类固醇、类黄酮、皂苷和酚类化合物的次级代谢产物。本研究采用1,1-二苯基-2-苦基肼(DPPH)进行自由基清除活性测定。通过使用Folin-Ciocalteau方法测试总酚含量的评估。结果表明,Sembung叶片的乙酸乙酯组分具有中等的抗氧化活性,IC50值为221.821,与IC50值68.25ppm的参考标准抗坏血酸相比。Sembung叶的乙酸乙酯部分的总酚含量为161.101mg GAE/g。本研究表明,苦瓜叶片具有抗氧化作用。
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