Rapid method for determining the antimicrobial susceptibility on chromogenic agar for urine specimen

Abstract

The traditional basis for the evaluation of urinary tract pathogens is the urine culture and the antibiotic susceptibility testing. The major drawback of the current microbiology approach is the time lapse of 2-3 days between specimen collection and the availability of the result with an objective evidence for treatment selection. Antibiotic resistance is a significant problem not only among nosocomial complicated UTIs, where it has traditionally been recognized, but also in community acquired simple UTIs. Early microbiological diagnosis and the correct administration of the appropriate initial antimicrobial therapy have proven to be associated with decrease rates of morbidity. Direct susceptibility testing of blood culture is being used in some microbiology laboratories, methods for urine cultures have been evaluated, some do not recommend the procedure, and others advocate a variety of conditions for acceptance of results. We performed a comparison between rapid method and standardized disk diffusion method in urine specimen, determining the antimicrobial susceptibility on CHROMagar Orientation Medium, without having to wait for bacterial isolation, our method may provide preliminary information that improves antimicrobial use and it also has a very good correlation with standard procedures. Methods