Screening of composite substrates suitable for tissue-cultured plantlets growth of Asparagus cochinchinensis (Lour.) Merr

IF 2.2 4区 农林科学 Q2 HORTICULTURE Folia Horticulturae Pub Date : 2022-03-07 DOI:10.2478/fhort-2022-0004
Xiaosong Yu, Lin Li, Yongquan Li, Z. Liu, N. Yang, Ming-sheng Zhang
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引用次数: 1

Abstract

Abstract Asparagus cochinchinensis (Lour.) Merr is a medicinal, edible and horticulture plant. Seedling breeding relies on tissue-cultured technology, but the transplant from tissue-cultured seedlings to field cultivation poses a problem in the large-scale production of A. cochinchinensis. Tissue-cultured seedlings of A. cochinchinensis were used as materials, and they were transplanted into the substrates that were mixed with coconut bran, peat, vermiculite, perlite or garden soil. The analysis of the physical and chemical properties of the substrate, combined with the survival rate of seedlings, the aboveground parameters (plant height, stem diameter, fresh weight and dry weight), underground parameters (root fresh weight, root dry weight, root length, root diameter, root surface area and root volume) and physiological indexes (malondialdehyde, soluble protein, soluble sugar, proline, chlorophyll and root vigour), allow us to understand the effects of different combination substrates on the growth and physiology of A. cochinchinensis tissue-cultured seedlings. We concluded that the survival rate of seedlings was negatively correlated with the bulk density and conductivity and was positively correlated with the porosity in the substrate. The membership function was used to comprehensively evaluate the indexes of each combination substrate, and it was concluded that coconut bran:perlite:vermiculite = 33.33%:33.33%:33.33% (bulk density = 0.23 g · cm−3, total porosity = 65.07%, pH = 6.30, conductivity = 0.51 mS · cm−1) scored the highest, the survival rate of seedlings reached 87.10%, the aboveground and underground growth were vigorous and the quality was better than other substrates. This research provides technical support for the efficient cultivation of tissue-cultured seedlings of A. cochinchinensis and reduces the cost of raising seedlings.
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适合南美白芦笋组织培养苗生长的复合基质的筛选
摘要芦笋是一种药用、食用和园艺植物。育苗依赖于组织培养技术,但从组织培养的幼苗移植到田间栽培,给胭脂虫的大规模生产带来了问题。以胭脂虫的组织培养苗为材料,将其移植到与椰子皮、泥炭、蛭石、珍珠岩或花园土壤混合的基质中。基质的理化性质分析,结合幼苗的存活率、地上参数(株高、茎径、鲜重和干重),地下参数(根鲜重、根干重、根长、根径、根表面积和根体积)和生理指标(丙二醛、可溶性蛋白、可溶性糖、脯氨酸、叶绿素和根系活力),使我们能够了解不同组合基质对胭脂虫组织培养苗生长和生理的影响。我们得出的结论是,幼苗的存活率与体积密度和电导率呈负相关,与基质中的孔隙率呈正相关。利用隶属函数对各组合基质的各项指标进行综合评价,得出椰子糠:珍珠岩:蛭石=33.33%:33.33%(容重=0.23 g·cm−3,总孔隙率=65.07%,pH=6.30,电导率=0.51 mS·cm−1)得分最高,幼苗成活率达87.10%,地上和地下生长旺盛,品质优于其他基质。本研究为高效培育胭脂虫组织培养苗提供了技术支持,降低了育苗成本。
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来源期刊
Folia Horticulturae
Folia Horticulturae Agricultural and Biological Sciences-Horticulture
CiteScore
3.40
自引率
0.00%
发文量
13
审稿时长
16 weeks
期刊介绍: Folia Horticulturae is an international, scientific journal published in English. It covers a broad research spectrum of aspects related to horticultural science that are of interest to a wide scientific community and have an impact on progress in both basic and applied research carried out with the use of horticultural crops and their products. The journal’s aim is to disseminate recent findings and serve as a forum for presenting views as well as for discussing important problems and prospects of modern horticulture, particularly in relation to sustainable production of high yield and quality of horticultural products, including their impact on human health.
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