Xiaosong Yu, Lin Li, Yongquan Li, Z. Liu, N. Yang, Ming-sheng Zhang
{"title":"Screening of composite substrates suitable for tissue-cultured plantlets growth of Asparagus cochinchinensis (Lour.) Merr","authors":"Xiaosong Yu, Lin Li, Yongquan Li, Z. Liu, N. Yang, Ming-sheng Zhang","doi":"10.2478/fhort-2022-0004","DOIUrl":null,"url":null,"abstract":"Abstract Asparagus cochinchinensis (Lour.) Merr is a medicinal, edible and horticulture plant. Seedling breeding relies on tissue-cultured technology, but the transplant from tissue-cultured seedlings to field cultivation poses a problem in the large-scale production of A. cochinchinensis. Tissue-cultured seedlings of A. cochinchinensis were used as materials, and they were transplanted into the substrates that were mixed with coconut bran, peat, vermiculite, perlite or garden soil. The analysis of the physical and chemical properties of the substrate, combined with the survival rate of seedlings, the aboveground parameters (plant height, stem diameter, fresh weight and dry weight), underground parameters (root fresh weight, root dry weight, root length, root diameter, root surface area and root volume) and physiological indexes (malondialdehyde, soluble protein, soluble sugar, proline, chlorophyll and root vigour), allow us to understand the effects of different combination substrates on the growth and physiology of A. cochinchinensis tissue-cultured seedlings. We concluded that the survival rate of seedlings was negatively correlated with the bulk density and conductivity and was positively correlated with the porosity in the substrate. The membership function was used to comprehensively evaluate the indexes of each combination substrate, and it was concluded that coconut bran:perlite:vermiculite = 33.33%:33.33%:33.33% (bulk density = 0.23 g · cm−3, total porosity = 65.07%, pH = 6.30, conductivity = 0.51 mS · cm−1) scored the highest, the survival rate of seedlings reached 87.10%, the aboveground and underground growth were vigorous and the quality was better than other substrates. This research provides technical support for the efficient cultivation of tissue-cultured seedlings of A. cochinchinensis and reduces the cost of raising seedlings.","PeriodicalId":12277,"journal":{"name":"Folia Horticulturae","volume":"34 1","pages":"39 - 50"},"PeriodicalIF":2.2000,"publicationDate":"2022-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Folia Horticulturae","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.2478/fhort-2022-0004","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"HORTICULTURE","Score":null,"Total":0}
引用次数: 1
Abstract
Abstract Asparagus cochinchinensis (Lour.) Merr is a medicinal, edible and horticulture plant. Seedling breeding relies on tissue-cultured technology, but the transplant from tissue-cultured seedlings to field cultivation poses a problem in the large-scale production of A. cochinchinensis. Tissue-cultured seedlings of A. cochinchinensis were used as materials, and they were transplanted into the substrates that were mixed with coconut bran, peat, vermiculite, perlite or garden soil. The analysis of the physical and chemical properties of the substrate, combined with the survival rate of seedlings, the aboveground parameters (plant height, stem diameter, fresh weight and dry weight), underground parameters (root fresh weight, root dry weight, root length, root diameter, root surface area and root volume) and physiological indexes (malondialdehyde, soluble protein, soluble sugar, proline, chlorophyll and root vigour), allow us to understand the effects of different combination substrates on the growth and physiology of A. cochinchinensis tissue-cultured seedlings. We concluded that the survival rate of seedlings was negatively correlated with the bulk density and conductivity and was positively correlated with the porosity in the substrate. The membership function was used to comprehensively evaluate the indexes of each combination substrate, and it was concluded that coconut bran:perlite:vermiculite = 33.33%:33.33%:33.33% (bulk density = 0.23 g · cm−3, total porosity = 65.07%, pH = 6.30, conductivity = 0.51 mS · cm−1) scored the highest, the survival rate of seedlings reached 87.10%, the aboveground and underground growth were vigorous and the quality was better than other substrates. This research provides technical support for the efficient cultivation of tissue-cultured seedlings of A. cochinchinensis and reduces the cost of raising seedlings.
期刊介绍:
Folia Horticulturae is an international, scientific journal published in English. It covers a broad research spectrum of aspects related to horticultural science that are of interest to a wide scientific community and have an impact on progress in both basic and applied research carried out with the use of horticultural crops and their products. The journal’s aim is to disseminate recent findings and serve as a forum for presenting views as well as for discussing important problems and prospects of modern horticulture, particularly in relation to sustainable production of high yield and quality of horticultural products, including their impact on human health.