Immunomodulatory activity of wild Artemisia rupestris L. crude polysaccharide as an adjuvant

国际生物医学工程杂志 Pub Date : 2019-10-28 DOI:10.3760/CMA.J.ISSN.1673-4181.2019.05.002

Quanxiao Li, Xueli Ba, Shuangshuang Feng, Ya-Li Tan, Bing Zhao, Xiaolong Luo

{"title":"Immunomodulatory activity of wild Artemisia rupestris L. crude polysaccharide as an adjuvant","authors":"Quanxiao Li, Xueli Ba, Shuangshuang Feng, Ya-Li Tan, Bing Zhao, Xiaolong Luo","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.05.002","DOIUrl":null,"url":null,"abstract":"Objective \nTo investigate the enhancement effect of Xinjiang wild Artemisia rupestris L. crude polysaccharides (WARCP) as an adjuvant on ovalbumin (OVA) vaccine in mice immunized intramuscularly. \n \n \nMethods \nICR mice were randomly divided into 6 groups (5 per group), including 9 g/L NaCl group (blank control), OVA group (10 μg OVA), low dose WARCP/OVA group (OVA+50 μg WARCP), medium dose WARCP/OVA group (OVA+200 μg WARCP), high dose WARCP/OVA group (OVA+400 μg WARCP), and aluminum adjuvant (Alum)/OVA group (positive control group, OVA+100 μg Alum). ICR mice were immunized intramuscularly and weighted. The OVA-specific antibodies and subtypes in serum were detected by enzyme linked immunosorbent assay (ELISA). T cells subsets from spleen and lymph nodes were detected by flow cytometry. \n \n \nResults \nThe medium-dose WARCP/OVA group enhanced IgG and IgG1 levels and increased early antibody levels (all P 0.05). The low-dose WARCP/OVA group enhanced the percentage of CD4+ T cells in spleen and CD4+ T, CD8+ T, CD4+CD44+ T cells in lymph nodes (all P<0.05). The medium dose WARCP/OVA group and the high dose WARCP/OVA group enhanced the CD4+ T, CD8+ T, CD4+CD44+ T, CD8+CD44+ T cells in spleen and CD8+CD44+ T cell in lymph nodes (all P<0.05). \n \n \nConclusions \nPlant-derived WARCP as an OVA protein vaccine adjuvant can enhance cellular immunity and humoral immunity, and it is safe and reliable. The results in this study provide a theoretical basis for the popularization and application of WARCP. \n \n \nKey words: \nWild Artemisia rupestris L.; Crude polysaccharides; Intramuscular immunization; Adjuvant","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2019-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"国际生物医学工程杂志","FirstCategoryId":"1087","ListUrlMain":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.05.002","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Objective To investigate the enhancement effect of Xinjiang wild Artemisia rupestris L. crude polysaccharides (WARCP) as an adjuvant on ovalbumin (OVA) vaccine in mice immunized intramuscularly. Methods ICR mice were randomly divided into 6 groups (5 per group), including 9 g/L NaCl group (blank control), OVA group (10 μg OVA), low dose WARCP/OVA group (OVA+50 μg WARCP), medium dose WARCP/OVA group (OVA+200 μg WARCP), high dose WARCP/OVA group (OVA+400 μg WARCP), and aluminum adjuvant (Alum)/OVA group (positive control group, OVA+100 μg Alum). ICR mice were immunized intramuscularly and weighted. The OVA-specific antibodies and subtypes in serum were detected by enzyme linked immunosorbent assay (ELISA). T cells subsets from spleen and lymph nodes were detected by flow cytometry. Results The medium-dose WARCP/OVA group enhanced IgG and IgG1 levels and increased early antibody levels (all P 0.05). The low-dose WARCP/OVA group enhanced the percentage of CD4+ T cells in spleen and CD4+ T, CD8+ T, CD4+CD44+ T cells in lymph nodes (all P<0.05). The medium dose WARCP/OVA group and the high dose WARCP/OVA group enhanced the CD4+ T, CD8+ T, CD4+CD44+ T, CD8+CD44+ T cells in spleen and CD8+CD44+ T cell in lymph nodes (all P<0.05). Conclusions Plant-derived WARCP as an OVA protein vaccine adjuvant can enhance cellular immunity and humoral immunity, and it is safe and reliable. The results in this study provide a theoretical basis for the popularization and application of WARCP. Key words: Wild Artemisia rupestris L.; Crude polysaccharides; Intramuscular immunization; Adjuvant

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

野生紫花蒿粗多糖作为佐剂的免疫调节作用

目的研究新疆野生黄花蒿粗多糖(WARCP)作为佐剂对小鼠肌内免疫卵清蛋白(OVA)疫苗的增强作用。方法将ICR小鼠随机分为6组(每组5只)，分别为9 g/L NaCl组(空白对照)、OVA组(10 μg OVA)、低剂量WARCP/OVA组(OVA+50 μg WARCP)、中剂量WARCP/OVA组(OVA+200 μg WARCP)、高剂量WARCP/OVA组(OVA+400 μg WARCP)、铝佐剂(明矾)/OVA组(阳性对照组，OVA+100 μg明矾)。对ICR小鼠进行肌肉免疫并称重。采用酶联免疫吸附试验(ELISA)检测血清中ova特异性抗体及其亚型。用流式细胞术检测脾脏和淋巴结的T细胞亚群。结果WARCP/OVA中剂量组血清IgG、IgG1水平升高，早期抗体水平升高(P < 0.05)。低剂量WARCP/OVA组脾脏CD4+ T细胞及淋巴结CD4+ T、CD8+ T、CD4+CD44+ T细胞百分比均升高(P<0.05)。WARCP/OVA中剂量组和WARCP/OVA高剂量组小鼠脾脏CD4+ T、CD8+ T、CD4+CD44+ T、CD8+CD44+ T细胞和淋巴结CD8+CD44+ T细胞均升高(P<0.05)。结论植物源性WARCP作为OVA蛋白疫苗佐剂可增强细胞免疫和体液免疫，安全可靠。研究结果为WARCP的推广应用提供了理论依据。关键词:野生黄花蒿;粗多糖;肌内免疫;辅助

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

国际生物医学工程杂志

自引率

0.00%

发文量

1974