Association of exposure to deoxynivalenol with DNA methylation in white blood cells in children in China

IF 1.7 4区医学 Q3 FOOD SCIENCE & TECHNOLOGY World Mycotoxin Journal Pub Date : 2021-10-22 DOI:10.3920/wmj2021.2699

P. Cao, X.D. Wang, J. Sun, J. Liang, P. Zhou, H. Xu, H. Yang, L. Zhang

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Abstract

Deoxynivalenol (DON) is a mycotoxin that commonly contaminates cereals worldwide. Dietary exposure to DON is a subject of great public health concern, but studies on the health effects of chronic exposure to DON are not available. In this study, we investigated the connection between DNA methylation levels and DON exposure in children. The DNA methylation status of white blood cells from 32 children aged 2~15 years old in Henan, China, was profiled. A total of 378 differentially methylated CpGs were identified between the high and low DON exposure groups, and 8 KEGG pathways were found to be significantly enriched among the differentially methylated genes. In addition, the quantitative methylation of EIF2AK4, EMID2 and GNASAS was analysed using the Sequenom MassARRAY platform. The results showed that the methylation level of EIF2AK4 was significantly different between the two groups, and the methylation levels were associated with exposure to DON. Conclusively, our study found that chronic exposure to DON during childhood could affect DNA methylation levels.

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中国儿童接触脱氧雪腐镰刀菌烯醇与白细胞DNA甲基化的关系

脱氧雪腐镰刀菌烯醇（DON）是一种常见于世界各地谷物的真菌毒素。DON的饮食暴露是一个备受公众健康关注的主题，但目前还没有关于长期接触DON对健康影响的研究。在这项研究中，我们调查了儿童DNA甲基化水平与DON暴露之间的联系。对河南地区32名2~15岁儿童白细胞DNA甲基化状况进行了分析。在高和低DON暴露组之间共鉴定出378个差异甲基化CpG，并且发现8个KEGG途径在差异甲基化基因中显著富集。此外，使用Sequenom MassARRAY平台分析了EIF2AK4、EMID2和GNASAS的定量甲基化。结果表明，EIF2AK4的甲基化水平在两组之间存在显著差异，并且甲基化水平与DON暴露有关。总之，我们的研究发现，儿童时期长期接触DON会影响DNA甲基化水平。

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来源期刊

World Mycotoxin Journal MYCOLOGY-

CiteScore

4.60

自引率

5.00%

发文量

审稿时长

>12 weeks

期刊介绍： ''World Mycotoxin Journal'' is a peer-reviewed scientific journal with only one specific area of focus: the promotion of the science of mycotoxins. The journal contains original research papers and critical reviews in all areas dealing with mycotoxins, together with opinions, a calendar of forthcoming mycotoxin-related events and book reviews. The journal takes a multidisciplinary approach, and it focuses on a broad spectrum of issues, including toxicology, risk assessment, worldwide occurrence, modelling and prediction of toxin formation, genomics, molecular biology for control of mycotoxigenic fungi, pre-and post-harvest prevention and control, sampling, analytical methodology and quality assurance, food technology, economics and regulatory issues. ''World Mycotoxin Journal'' is intended to serve the needs of researchers and professionals from the scientific community and industry, as well as of policy makers and regulators.