Association of vitamin D receptor gene polymorphism (rs10735810) and chronic periodontitis

Q1 Medicine Journal of Investigative and Clinical Dentistry Pub Date : 2019-07-19 DOI:10.1111/jicd.12440

Karthikeyan Murthykumar, Radhika Arjunkumar, Vijayashree Priyadharsini Jayaseelan

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引用次数: 31

Abstract

Aim

The aim of the present study was to analyze the association between vitamin D receptor (VDR) (rs10735810) gene polymorphism and chronic periodontitis (CP).

Methods

A total of 100 subjects were recruited for this study, which included 50 CP and 50 healthy controls. Genomic DNA was extracted from the whole blood collected from the subjects. DNA was amplified using specific primers flanking the FokI region of the VDR gene (rs10735810). The amplicon was further subjected to genotyping using restriction fragment length polymorphism (RFLP) using the FokI enzyme. The genotype obtained based on RFLP pattern was recorded and used for statistical analysis. The distribution of genotypes and allele frequencies in the chronic periodontitis and control groups were compared using the χ²-test.

Results

The CP group displayed the highest frequency of CT (20%) and TT (6%) genotypes when compared with the control subjects. Allele frequency was found to be similar in both groups. The C allele was found to be predominant in the study population compared with the T allele.

Conclusion

The present study denotes that the VDR polymorphism (rs10735810) is not associated with CP in the study group analyzed.

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维生素D受体基因多态性(rs10735810)与慢性牙周炎的关系

目的分析维生素D受体(VDR) (rs10735810)基因多态性与慢性牙周炎(CP)的关系。方法共招募100例受试者，其中CP 50例，健康对照50例。基因组DNA是从受试者采集的全血中提取的。利用VDR基因(rs10735810) FokI区两侧的特异性引物扩增DNA。利用FokI酶进一步利用限制性片段长度多态性(RFLP)对扩增子进行基因分型。记录基于RFLP模式得到的基因型并进行统计分析。慢性牙周炎患者与对照组基因型及等位基因频率分布比较，采用χ2检验。结果与对照组相比，CP组CT(20%)和TT(6%)基因型发生率最高。发现两组的等位基因频率相似。与T等位基因相比，C等位基因在研究人群中占优势。结论VDR多态性(rs10735810)在研究人群中与CP无关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Investigative and Clinical Dentistry DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.90

自引率

0.00%

发文量

期刊介绍： Journal of Investigative and Clinical Dentistry (JICD) aims to publish quality, peer reviewed original research and topical reviews on all aspects of investigative and clinical dentistry and craniofacial research, including molecular studies related to oral health and disease. Although international in outlook, the Editor especially encourages papers from the Asia Pacific. The journal also aims to provide clinicians, scientists and students of dentistry with a knowledge transfer platform for rapid publication of reports through an international journal, which will be available free online until 2012. Its scope, therefore, is broad, inclusive and international, but with a particular focus on Asia Pacific. The Editor welcomes manuscripts in the following key thematic areas in oral and maxillofacial sciences: Community Dentistry and Oral Epidemiology, Conservative Dentistry, Dental Biomaterials, Dental Pedagogy, Endodontics and Traumatology, Implant Dentistry, Oral Biosciences, Oral and Maxillofacial Surgery, Oral Medicine, Oral Pathology and Oral Microbiology, Orthodontics, Oral Radiology, Oral Rehabilitation, Paedodontics, Periodontology and Periodontal Medicine.

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