In vitro mass multiplication of economically endangered forest plant Chironji (Buchanania lanzan Spreng.)

IF 1 4区 生物学 Q3 PLANT SCIENCES Indian Journal of Genetics and Plant Breeding Pub Date : 2022-02-25 DOI:10.31742/ijgpb.82.1.18
Swapnil Singh, Radheshyam Sharma, R. Ramakrishnan, Stuti Sharma, N. Dwivedi, Ashish Kumar, V. S. Gaur
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Abstract

A protocol was developed for organogenesis and in vitro multiplication of chironji using young leaf and nodal segments. Maximum 41 callus were induced in MS containing 2.5mg/l 2,4-D after 3 weeks of inoculations of leaf explants. Further, callus and nodal segments were inoculated alone and combination of Thidiazuron (TDZ), BAP, and Kinetin and the maximum shoot induction was obtained in a wood plant medium 2.5mg/L TDZ alone enriched with 0.1% of activated charcoal. In comparison, the maximum shoot proliferation (78%) was observed in ½WPM containing 2.5mg/L TDZ and 0.5mg/L GA3 with 5.7 shoots per explants. Maximum 8 roots were observed in vitro regenerated shoots with WPM supplemented with 2.0 mg/l IBA enriched with 0.2% activated charcoal. Plants with 3-4cm in root length were acclimatized and transferred to pots containing an autoclaved mixture of soil, sand and manure in 2:1:1 ratio. Approximately 70% survival rate was recorded from in vitro grown plantlets on transfer to pots.
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经济濒危森林植物Chironji(Buchanania lanzan Spreng.)的体外大规模繁殖
开发了一种利用幼叶和节段进行chironji器官发生和体外繁殖的方案。叶片外植体接种3周后,在含有2.5mg/l 2,4-D的MS中最多可诱导41个愈伤组织。此外,在单独添加0.1%活性炭的2.5mg/L TDZ的木质植物培养基中,单独接种Thidiazuron(TDZ)、BAP和Kinetin组合的愈伤组织和节段,获得了最大的芽诱导。相比之下,在含有2.5mg/L TDZ和0.5mg/L GA3的½WPM中观察到最大的芽增殖(78%),每个外植体有5.7个芽。在添加有2.0mg/l IBA(富含0.2%活性炭)的WPM的体外再生芽中,观察到最多8个根。将根长为3-4cm的植物驯化,并将其转移到含有土壤、沙子和肥料的高压灭菌混合物(比例为2:1:1)的花盆中。从体外培养的植株转移到花盆中,记录到大约70%的存活率。
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来源期刊
CiteScore
1.80
自引率
10.00%
发文量
0
审稿时长
6-12 weeks
期刊介绍: Advance the cause of genetics and plant breeding and to encourage and promote study and research in these disciplines in the service of agriculture; to disseminate the knowledge of genetics and plant breeding; provide facilities for association and conference among students of genetics and plant breeding and for encouragement of close relationship between them and those in the related sciences; advocate policies in the interest of the nation in the field of genetics and plant breeding, and facilitate international cooperation in the field of genetics and plant breeding.
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