Swapnil Singh, Radheshyam Sharma, R. Ramakrishnan, Stuti Sharma, N. Dwivedi, Ashish Kumar, V. S. Gaur
{"title":"In vitro mass multiplication of economically endangered forest plant Chironji (Buchanania lanzan Spreng.)","authors":"Swapnil Singh, Radheshyam Sharma, R. Ramakrishnan, Stuti Sharma, N. Dwivedi, Ashish Kumar, V. S. Gaur","doi":"10.31742/ijgpb.82.1.18","DOIUrl":null,"url":null,"abstract":"A protocol was developed for organogenesis and in vitro multiplication of chironji using young leaf and nodal segments. Maximum 41 callus were induced in MS containing 2.5mg/l 2,4-D after 3 weeks of inoculations of leaf explants. Further, callus and nodal segments were inoculated alone and combination of Thidiazuron (TDZ), BAP, and Kinetin and the maximum shoot induction was obtained in a wood plant medium 2.5mg/L TDZ alone enriched with 0.1% of activated charcoal. In comparison, the maximum shoot proliferation (78%) was observed in ½WPM containing 2.5mg/L TDZ and 0.5mg/L GA3 with 5.7 shoots per explants. Maximum 8 roots were observed in vitro regenerated shoots with WPM supplemented with 2.0 mg/l IBA enriched with 0.2% activated charcoal. Plants with 3-4cm in root length were acclimatized and transferred to pots containing an autoclaved mixture of soil, sand and manure in 2:1:1 ratio. Approximately 70% survival rate was recorded from in vitro grown plantlets on transfer to pots.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0000,"publicationDate":"2022-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indian Journal of Genetics and Plant Breeding","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.31742/ijgpb.82.1.18","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
A protocol was developed for organogenesis and in vitro multiplication of chironji using young leaf and nodal segments. Maximum 41 callus were induced in MS containing 2.5mg/l 2,4-D after 3 weeks of inoculations of leaf explants. Further, callus and nodal segments were inoculated alone and combination of Thidiazuron (TDZ), BAP, and Kinetin and the maximum shoot induction was obtained in a wood plant medium 2.5mg/L TDZ alone enriched with 0.1% of activated charcoal. In comparison, the maximum shoot proliferation (78%) was observed in ½WPM containing 2.5mg/L TDZ and 0.5mg/L GA3 with 5.7 shoots per explants. Maximum 8 roots were observed in vitro regenerated shoots with WPM supplemented with 2.0 mg/l IBA enriched with 0.2% activated charcoal. Plants with 3-4cm in root length were acclimatized and transferred to pots containing an autoclaved mixture of soil, sand and manure in 2:1:1 ratio. Approximately 70% survival rate was recorded from in vitro grown plantlets on transfer to pots.
期刊介绍:
Advance the cause of genetics and plant breeding and to encourage and promote study and research in these disciplines in the service of agriculture; to disseminate the knowledge of genetics and plant breeding; provide facilities for association and conference among students of genetics and plant breeding and for encouragement of close relationship between them and those in the related sciences; advocate policies in the interest of the nation in the field of genetics and plant breeding, and facilitate international cooperation in the field of genetics and plant breeding.