A semi-quantitative multi-range lateral flow immunoassay for amantadine residues in livestock and poultry products

IF 1.7 3区农林科学 Q3 CHEMISTRY, APPLIED Food and Agricultural Immunology Pub Date : 2022-10-30 DOI:10.1080/09540105.2022.2137472

Xiangxue Ning, Jing Qiu

引用次数: 0

Abstract

ABSTRACT Amantadine is one of the most prescribed antiviral medications. Considering its abuse is life-threatening, monitoring amantadine residues is urgently needed. This study designed a dipstick assay based on lateral flow immunoassay (LFIA) platforms with an indicator range of 50–200 ng/mL. The dipstick consists of four coloured test lines that detect the concentration of amantadine semi-quantitatively in the range of 0–200 ng/mL. The amantadine concentration was categorised as 0–50, 50–100, 100–200, or > 200 ng/mL, depending on the response of the test lines. Furthermore, we measured the amantadine residues in commercial meat samples by this LFIA. The results were highly agreeable with those determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The LFIA shows potential for routine monitoring of amantadine in meat products, and the results of this study provide insight into the development of new LFIA devices for use in agricultural industries.

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畜禽产品中金刚烷胺残留的半定量多量程横向流动免疫分析

摘要：金刚烷胺是处方最多的抗病毒药物之一。考虑到金刚烷胺的滥用会危及生命，因此迫切需要监测金刚烷胺残留情况。本研究设计了一种基于侧流免疫测定（LFIA）平台的试纸测定法，其指标范围为50-200 ng/mL。量油尺由四条彩色测试线组成，可半定量检测0–200范围内的金刚烷胺浓度 ng/mL。金刚烷胺的浓度分为0-50、50-100、100-200或>200 ng/mL，取决于测试线的响应。此外，我们还通过LFIA测定了商业肉类样品中金刚烷胺的残留量。结果与液相色谱-串联质谱法（LC-MS/MS）测定结果一致。LFIA显示出对肉制品中金刚烷胺进行常规监测的潜力，本研究的结果为开发用于农业的新型LFIA设备提供了见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Food and Agricultural Immunology 农林科学-毒理学

CiteScore

5.30

自引率

6.70%

发文量

审稿时长

2 months

期刊介绍： Food and Agricultural Immunology is an international open access journal publishing original immunological research with applications in food, agricultural, environmental and veterinary science. Submissions describing the use of immunological techniques and methods are particularly welcomed. The journal aims to expand our understanding of the interactions at the interface of food and immune systems including studies on: -Development of diagnostic systems – all types of ligand-based assays, e.g. antibody, aptamer -Application of ligand-based assays for the detection or identification of molecules of interest in food science, agricultural research, veterinary investigations and clinical systems relating to food allergy or sensitivity to agricultural chemicals -Effects of food on the immune system -Studies on allergy and allergic reactions -Investigations into food allergies -Development of allergen-free food systems -Development of novel assay formats -Applications of assay systems to the monitoring of food items in relation to safety and labelling -Food quality issues, e.g. speciation, adulteration and contamination -Comparisons between different analytical techniques The journal publishes research and review articles and is essential reading for food scientists, immunologists and all those concerned with the interaction between food and immune systems.