Comparison of methods to recover amaranth weed seeds from manure

IF 2.3 4区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Agricultural & Environmental Letters Pub Date : 2022-01-30 DOI:10.1002/ael2.20065
Melissa L. Wilson, Anthony Brusa, Hatley Christensen, Samuel Strack, Eddie Alto, Luis F. Allen, Scott D. Cortus, Chryseis Modderman, Roger L. Becker
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Abstract

One pathway by which Palmer amaranth (Amaranthus palmeri S. Watson) invades new areas is through importation of contaminated livestock feed, which then contaminates land-applied manure. If contaminated feed is suspected, detection tools are needed to test manure, but traditional methods are time consuming and often inconclusive. Although new genetic seed testing is making detection easier, methods to separate seed from contaminated manure are needed. Six methods were compared for their ability to recover 100 Palmer amaranth seeds added to bedded or nonbedded cattle manure: dry sieving, rinse sieving, manure saturation sieving without blending and with blending, and dispersion sieving without blending and with blending. Seed recovery was highest (>90%) with the rinse sieving method regardless of manure type. The dispersion methods are not recommended as they recovered <24.7% of seeds. Following each method, genetic testing successfully identified Palmer amaranth presence, indicating no interference of recovery method with DNA extraction.

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从粪便中回收苋菜杂草种子的方法比较
Palmer amaranth(Amaranthus palmeri S.Watson)入侵新地区的一条途径是进口受污染的牲畜饲料,然后污染土地施用的肥料。如果怀疑饲料受到污染,就需要检测工具来检测粪便,但传统的方法很耗时,而且往往没有结论。尽管新的基因种子检测使检测变得更容易,但仍需要从受污染的粪便中分离种子的方法。比较了六种方法回收添加到有床或无床牛粪中的100粒帕尔默苋种子的能力:干筛、漂洗筛、无混合和有混合的粪肥饱和筛,以及无混合和混合的分散筛。无论粪肥类型如何,冲洗筛分法的种子回收率最高(>90%)。不推荐使用分散方法,因为它们回收的种子少于24.7%。在每种方法之后,基因测试都成功地确定了Palmer苋的存在,表明回收方法对DNA提取没有干扰。
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CiteScore
3.70
自引率
3.80%
发文量
28
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