Methicillin and Mupirocin resistance genes in Staphylococcus aureus isolated from hospital staff in Darab by using of Multiplex

Abstract

and Mupirocin –resistance (MRSA) are considered as the important hospital pathogens. One of the important resources of this organism in nosocomial infection is related to hospital personnel. The objective of this research was is to investigate the frequency of MRSA gene in staphylococcus aureus isolated from Hospital staff in Darab by multiplex PCR. In this study, 150 hospital staffs were investigated and specimens of staphylococcus aureus which isolated from those were confirmed by biochemical and microbial methods. The disk diffusion method was used to determine the susceptibility of the isolates to methicillin and mupirocin antibiotic. Then, the presence of mec-A, mup and nuc genes were investigated by using of Multiplex PCR method. of Hospital was relatively low. However, permanent control of the carriers and treatment of them can prevent the spread of this bacterium and its associated infections.