{"title":"DNA Damage and Repair in different Tissues of Fresh Water Fish, Channa punctata after Acute and Subchronic Exposure to bisphenol A","authors":"Prince Sharma, K. Sharma, P. Chadha","doi":"10.18311/ti/2022/v29i3/28352","DOIUrl":null,"url":null,"abstract":"The present study was conducted to investigate the genotoxic effect of Bis-Phenol A (BPA) after acute and subchronic exposure in different tissues of Channa punctata. The recovery in DNA damage was also ascertained after 30 days of cessation of exposure. Fish were exposed to different sublethal concentrations of BPA along with two controls i.e., with positive (acetone) and negative (water) controls for 96h (acute exposure) and 60 days (subchronic exposure) and after that fish were allowed to recover for 30 days in freshwater. The blood, liver, and gill tissue samples were collected at 24, 48, 72 and 96h for acute exposure and after 20, 40, and 60 days post-exposure for subchronic exposure. Exposed groups showed significantly higher DNA damage in both acute and subchronic exposure as compared to control groups. In the case of acute exposure, the highest damage was observed at 24 h of exposure followed by a decline in the value of all the parameters, while in the later hours of exposure these values further increased. On the other hand, in the case of sub-chronic exposure, the highest damage was observed after 60 days of exposure. Recovery experiment showed a decrease in the values of all the parameters studied. The result of the study clearly showed that BPA caused DNA damage in Channa punctata after acute as well as subchronic exposure.","PeriodicalId":23205,"journal":{"name":"Toxicology International","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Toxicology International","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18311/ti/2022/v29i3/28352","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Pharmacology, Toxicology and Pharmaceutics","Score":null,"Total":0}
引用次数: 0
Abstract
The present study was conducted to investigate the genotoxic effect of Bis-Phenol A (BPA) after acute and subchronic exposure in different tissues of Channa punctata. The recovery in DNA damage was also ascertained after 30 days of cessation of exposure. Fish were exposed to different sublethal concentrations of BPA along with two controls i.e., with positive (acetone) and negative (water) controls for 96h (acute exposure) and 60 days (subchronic exposure) and after that fish were allowed to recover for 30 days in freshwater. The blood, liver, and gill tissue samples were collected at 24, 48, 72 and 96h for acute exposure and after 20, 40, and 60 days post-exposure for subchronic exposure. Exposed groups showed significantly higher DNA damage in both acute and subchronic exposure as compared to control groups. In the case of acute exposure, the highest damage was observed at 24 h of exposure followed by a decline in the value of all the parameters, while in the later hours of exposure these values further increased. On the other hand, in the case of sub-chronic exposure, the highest damage was observed after 60 days of exposure. Recovery experiment showed a decrease in the values of all the parameters studied. The result of the study clearly showed that BPA caused DNA damage in Channa punctata after acute as well as subchronic exposure.
期刊介绍:
Toxicology International is a peer-reviewed International Research Journal published bi-annually by the Society of Toxicology, India. The Journal is concerned with various disciplines of Toxicology including man, animals, plants and environment and publishes research, review and general articles besides opinions, comments, news-highlights and letters to editor.