Evaluation of Aspirin Effect on Candida Glabrata Isolates with Resistance to Azole Compounds by Real-Time PCR

F. Nojoomi, M. Vafaei, F. Bagheri, Zohreh Zamanian
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Abstract

Materials and methods: Five C. glabrata isolates were collected from patients with candidal vulvovaginitis referred to clinic. The antibiotic susceptibility test of isolates for fluconazole was performed according to the CLSI M38-A2 standard protocol. A 64μg/ml aspirin and 500ug/ml and likewise 64μg/ml fluconazole were used for the MIC detection test. The RNA of isolates was prepared according to the manufacturer’s instruction. Synthesis of cDNA was conducted with the 1621K kit (Fermentase).. The RT-PCR reaction was set up for ERG3, ERG6 and ERG11 genes and the housekeeping gene Beta-actin (β-actin) was amplified as internal control.
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实时聚合酶链反应评价阿司匹林对含氮化合物耐药念珠菌的作用
材料与方法:从临床转诊的念珠菌性外阴阴道炎患者中分离得到5株光滑念珠菌。分离株对氟康唑的抗生素敏感性试验按照CLSI M38-A2标准方案进行。MIC检测试验使用64μg/ml阿司匹林和500μg/ml氟康唑,同样使用64μg/ml氟康唑。根据制造商的说明制备分离物的RNA。cDNA的合成是用1621K试剂盒(发酵酶)进行的。。建立ERG3、ERR6和ERG11基因的RT-PCR反应,扩增管家基因β-肌动蛋白(β-actin)作为内部对照。
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