Phytochemical Investigation of Hypericum heterophyllum Flowers: LC-ESI-MS/MS Analysis, Total Phenolic and Flavonoid Contents, Antioxidant Activity

IF 0.7 Q4 CHEMISTRY, MEDICINAL Natural Products Journal Pub Date : 2023-01-12 DOI:10.2174/2210315513666230112165545

R. Erenler, C. Yaman, lbrahim Demirtas, M. Hakkı Alma

{"title":"Phytochemical Investigation of Hypericum heterophyllum Flowers: LC-ESI-MS/MS Analysis, Total Phenolic and Flavonoid Contents, Antioxidant Activity","authors":"R. Erenler, C. Yaman, lbrahim Demirtas, M. Hakkı Alma","doi":"10.2174/2210315513666230112165545","DOIUrl":null,"url":null,"abstract":"\n\nHypericum heterophyllum has been used as a traditional medicine and includes significant bioactive compounds.\n\n\n\nTo quantitatively determine the phenolic and flavonoid contents of Hypericum heterophyllum flowers and evaluate the antioxidant activity. Also, to present the activity-compound relationship.\n\n\n\nFlowers of H. heterophyllum Vent., endemic species for Turkey were collected from Yozgat-Turkey. H. heterophyllum flowers were extracted with methanol, ethanol, acetone, and chloroform individually. LC-ESI-MS/MS analysis for phytochemicals was carried out for each extract. Total phenolic and flavonoid content were carried out for each extract. The antioxidant activity was performed using DPPH•, ABTS•+, hydroxyl radical, and superoxide radical scavenging assays.\n\n\n\nChlorogenic acid was found as a major compound in all extracts. Among the extracts, methanol extract included the most phenolic compound. The methanol extract and acetone extract consisted of the most flavonoids. Acetone and ethanol extracts revealed the most DPPH and ABTS activity respectively. Acetone extract exhibited the highest hydroxyl radical and superoxide radical scavenging effect\n\n\n\nFlowers of H. heterophyllum contain bioactive compounds and they revealed good antioxidant activity. Hence, H. heterophyllum flowers could be a promising agent for the pharmaceutical and food industries.\n","PeriodicalId":56153,"journal":{"name":"Natural Products Journal","volume":null,"pages":null},"PeriodicalIF":0.7000,"publicationDate":"2023-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Natural Products Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2174/2210315513666230112165545","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, MEDICINAL","Score":null,"Total":0}

引用次数: 3

Abstract

Hypericum heterophyllum has been used as a traditional medicine and includes significant bioactive compounds. To quantitatively determine the phenolic and flavonoid contents of Hypericum heterophyllum flowers and evaluate the antioxidant activity. Also, to present the activity-compound relationship. Flowers of H. heterophyllum Vent., endemic species for Turkey were collected from Yozgat-Turkey. H. heterophyllum flowers were extracted with methanol, ethanol, acetone, and chloroform individually. LC-ESI-MS/MS analysis for phytochemicals was carried out for each extract. Total phenolic and flavonoid content were carried out for each extract. The antioxidant activity was performed using DPPH•, ABTS•+, hydroxyl radical, and superoxide radical scavenging assays. Chlorogenic acid was found as a major compound in all extracts. Among the extracts, methanol extract included the most phenolic compound. The methanol extract and acetone extract consisted of the most flavonoids. Acetone and ethanol extracts revealed the most DPPH and ABTS activity respectively. Acetone extract exhibited the highest hydroxyl radical and superoxide radical scavenging effect Flowers of H. heterophyllum contain bioactive compounds and they revealed good antioxidant activity. Hence, H. heterophyllum flowers could be a promising agent for the pharmaceutical and food industries.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

金丝桃花的植物化学研究:LC-ESI-MS/MS分析、总酚和类黄酮含量、抗氧化活性

异叶金丝桃是一种具有重要生物活性的传统药材。目的定量测定异叶金丝桃花中酚类和类黄酮的含量，并评价其抗氧化活性。同时，给出了活性-化合物关系。异叶蓼的花。，土耳其特有种采集自土耳其约兹加特。分别用甲醇、乙醇、丙酮和氯仿提取异叶参花。对各提取物进行LC-ESI-MS/MS分析。测定各提取物的总酚和类黄酮含量。通过DPPH•、ABTS•+、羟基自由基和超氧自由基清除实验来检测其抗氧化活性。绿原酸是所有提取物中的主要化合物。其中，甲醇提取物含酚类化合物最多。甲醇提取物和丙酮提取物中黄酮类化合物含量最高。丙酮和乙醇提取物的DPPH和ABTS活性最高。丙酮提取物对羟基自由基和超氧自由基的清除作用最强，花中含有生物活性物质，具有良好的抗氧化活性。因此，在制药和食品工业中，异叶水仙花可能是一种很有前途的药剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Natural Products Journal CHEMISTRY, MEDICINAL-

CiteScore

1.70

自引率

0.00%

发文量

期刊介绍： The Natural Products Journal a peer reviewed journal, aims to publish all the latest and outstanding developments in natural products. The Natural Products Journal publishes original research articles, full-length/mini reviews, letters and guest edited issues on all aspects of research and development in the field including: isolation, purification, structure elucidation, synthesis and bioactivity of chemical compounds found in nature.