Concentrations of neopterin, kynurenine and tryptophan in wound secretions of patients with breast cancer and malignant melanoma: a pilot study

IF 0.5 4区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pteridines Pub Date : 2017-12-20 DOI:10.1515/pterid-2017-0018

J. Mergancová, L. Javorská, J. Šiller, L. Sákra, J. Mergancová, D. Solichová, L. Krčmová, B. Melichar, B. Mohelníková-Duchoňová, H. Študentová, D. Klos

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引用次数: 1

Abstract

Abstract The aim of the present pilot study was to investigate the concentrations of neopterin, kynurenine and tryptophan in wound secretion in patients undergoing surgery for breast cancer or malignant melanoma. Twenty-two patients, 16 females and 6 males, undergoing surgery for breast cancer (n=15) or malignant melanoma (n=7) were evaluated. Neopterin, kynurenine and tryptophan were determined using a high-performance liquid chromatography method. When the concentrations in wound secretions from the primary breast tumor and the axilla were compared, the neopterin/tryptophan ratio was significantly higher in the tumor wound secretions (0.92±0.41 vs. 0.61±0.14 mmol/mol; p=0.049), but no significant differences were observed in neopterin (49.2±28.6 vs. 31.5±11.1 nmol/L), tryptophan (52.9±13.0 vs. 51.2±13.3 μmol/L) and kynurenine concentrations (5.97±7.49 vs. 5.34±6.25 μmol/L) and kynurenine/tryptophan ratio (108.1±107.7 vs. 103.5±106.7 mmol/mol). No marked differences were noted in neopterin, tryptophan and kynurenine concentrations and kynurenine/tryptophan and neopterin/tryptophan ratios in sequential samples from the axilla of breast cancer patients obtained on days 1 and 2. In conclusion, present data demonstrate that the measurement of neopterin, kynurenine and tryptophan can be used to monitor local immune response after cancer surgery.

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乳腺癌和恶性黑色素瘤患者伤口分泌物中新蝶呤、犬尿氨酸和色氨酸浓度的初步研究

摘要本初步研究的目的是研究乳腺癌或恶性黑色素瘤手术患者伤口分泌物中新蝶呤、犬尿氨酸和色氨酸的浓度。22例因乳腺癌(n=15)或恶性黑色素瘤(n=7)接受手术的患者，其中女性16例，男性6例。采用高效液相色谱法测定新蝶呤、犬尿氨酸和色氨酸。比较原发乳腺肿瘤和腋窝创面分泌物中新蝶呤/色氨酸的浓度，肿瘤创面分泌物中新蝶呤/色氨酸的比值(0.92±0.41∶0.61±0.14 mmol/mol)显著高于腋窝创面分泌物(0.61±0.14 mmol/mol);p=0.049)，但新蝶呤(49.2±28.6 vs. 31.5±11.1 nmol/L)、色氨酸(52.9±13.0 vs. 51.2±13.3 μmol/L)、犬尿氨酸浓度(5.97±7.49 vs. 5.34±6.25 μmol/L)和犬尿氨酸/色氨酸比值(108.1±107.7 vs. 103.5±106.7 mmol/mol)差异无统计学意义。在第1天和第2天获得的乳腺癌患者腋窝连续样品中，新蝶呤、色氨酸和犬尿氨酸浓度以及犬尿氨酸/色氨酸和新蝶呤/色氨酸比率没有明显差异。总之，目前的数据表明，测量新蝶呤、犬尿氨酸和色氨酸可用于监测肿瘤手术后局部免疫反应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pteridines 生物-生化与分子生物学

CiteScore

1.20

自引率

25.00%

发文量

审稿时长

>12 weeks

期刊介绍： Pteridines is an open acess international quarterly journal dealing with all aspects of pteridine research. Pteridines are heterocyclic fused ring compounds involved in a wide range of biological functions from the color on butterfly wings to cofactors in enzyme catalysis to essential vitamins. Of the pteridines, 5,6,7,8-tetrahydrobiopterin is the necessary cofactor of several aromatic amino acid monoxygenases, the nitric oxide synthases and glyceryl ether monoxygenase (GEMO). Neopterin plays an essential role in the immune system and is an important biomarker in laboratory medicine for diseases such as HIV, cardiovascular disease, malignant tumors, among others. Topics: -Neopterin, dihydroneopterin, monapterin- Biopterin, tetrahydrobiopterin- Folates, antifolates, riboflavin- Phenylalanine, tyrosine, phenylketonuria, serotonin, adrenalin, noradrenalin, L-DOPA, dopamine, related biogenic amines- Phenylalanine hydroxylase, tyrosine hydroxylase, tryptophan hydroxylase, nitric oxide synthases (iNOS), alkylglycerol monooxygenase (AGMO), dihydropterin reductase, sepiapterin reductase- Homocysteine, mediators of inflammation, redox systems, iron.