Sheep IGFBP2 and IGFBP4 promoter methylation regulates gene expression and hair follicle development

IF 2.3 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Electronic Journal of Biotechnology Pub Date : 2022-09-01 DOI:10.1016/j.ejbt.2022.07.001

Yuezhen Tian , Jianwen Du , Xuemei Yang , Weidan Zeng , Junmin He , Bingru Zhao , Xuefeng Fu , Xinming Xu , Weiwei Wu , Jiang Di , Xixia Huang , Kechuan Tian

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引用次数: 3

Abstract

Background

Hair follicle development is important for the fineness of wool. Our previous methylated DNA immunoprecipitation sequencing showed that IGFBP2 and IGFBP4 were differentially methylated at different development stages of the hair follicle of Super Merino sheep. This study selected these two genes as candidates to uncover the influence of their promoter methylation on hair follicle development.

Results

The results showed that the expression levels of IGFBP2 and IGFBP4 in the skin of very fine wool Super Merino sheep were higher than that in the very thick wool group, and the difference for IGFBP4 was significant. The total methylation rate of IGFBP2 promoter was extremely low, and no significant difference was found in the skin tissue of the Super Merino sheep at different fineness groups. However, the total methylation rate of IGFBP4 promoter in the very thick group was significantly higher than that in the very fine group, and the total DNA methylation levels of the IGFBP4 were significantly negatively correlated with mRNA expression. Furthermore, the transcription factor prediction results showed 104 and 91 candidate binding sites for transcription factors in the promoter regions of IGFBP2 and IGFBP4, respectively. Among these, JUN, EP300, SP1, PAX6, ETS1, and MYOD1 may be related to hair follicle development.

Conclusions

The total methylation level of IGFBP4 promoter was negatively correlated with its mRNA expression in Super Merino sheep skin tissues with different degrees of fineness, which could be used as an epigenetic marker for wool breeding in Super Merino sheep.

How to cite: Tian Y, Du J, Yang X, et al. Sheep IGFBP2 and IGFBP4 promoter methylation regulates gene expression and hair follicle development. Electron J Biotechnol 2022;59. https://doi.org/10.1016/j.ejbt.2022.07.001.

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绵羊IGFBP2和IGFBP4启动子甲基化调节基因表达和毛囊发育

毛囊的发育对羊毛的细度很重要。我们之前的甲基化DNA免疫沉淀测序显示，IGFBP2和IGFBP4在超级美利奴羊毛囊的不同发育阶段存在差异甲基化。本研究选择这两个基因作为候选基因，以揭示其启动子甲基化对毛囊发育的影响。结果超细羊毛超级美利奴羊皮肤中IGFBP2和IGFBP4的表达量高于超厚羊毛组，其中IGFBP4表达量差异显著。IGFBP2启动子总甲基化率极低，不同细度组超级美利奴羊皮肤组织中IGFBP2启动子总甲基化率无显著差异。但极厚组IGFBP4启动子总甲基化率显著高于极细组，且IGFBP4 DNA总甲基化水平与mRNA表达呈显著负相关。此外，转录因子预测结果显示，IGFBP2和IGFBP4的启动子区域分别有104个和91个候选转录因子结合位点。其中JUN、EP300、SP1、PAX6、ETS1、MYOD1可能与毛囊发育有关。结论在不同细度的超级美利奴绵羊皮肤组织中，IGFBP4启动子总甲基化水平与其mRNA表达呈负相关，可作为超级美利奴绵羊羊毛育种的表观遗传标记。引用方式:田毅，杜军，杨旭，等。绵羊IGFBP2和IGFBP4启动子甲基化调节基因表达和毛囊发育。中国生物医学工程学报(英文版);2011;https://doi.org/10.1016/j.ejbt.2022.07.001。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Electronic Journal of Biotechnology 工程技术-生物工程与应用微生物

CiteScore

5.60

自引率

0.00%

发文量

审稿时长

2 months

期刊介绍： Electronic Journal of Biotechnology is an international scientific electronic journal, which publishes papers from all areas related to Biotechnology. It covers from molecular biology and the chemistry of biological processes to aquatic and earth environmental aspects, computational applications, policy and ethical issues directly related to Biotechnology. The journal provides an effective way to publish research and review articles and short communications, video material, animation sequences and 3D are also accepted to support and enhance articles. The articles will be examined by a scientific committee and anonymous evaluators and published every two months in HTML and PDF formats (January 15th , March 15th, May 15th, July 15th, September 15th, November 15th). The following areas are covered in the Journal: • Animal Biotechnology • Biofilms • Bioinformatics • Biomedicine • Biopolicies of International Cooperation • Biosafety • Biotechnology Industry • Biotechnology of Human Disorders • Chemical Engineering • Environmental Biotechnology • Food Biotechnology • Marine Biotechnology • Microbial Biotechnology • Molecular Biology and Genetics •Nanobiotechnology • Omics • Plant Biotechnology • Process Biotechnology • Process Chemistry and Technology • Tissue Engineering