Evaluation of Stryphnodendron polyphyllum (Mart.) pollen as antioxidant and antibacterial potential

Rafael Nogueira Oliveira Albuquerque, C. H. G. Martins, Jonathan Henrique de Souza, V. D. M. Sacramento, D. Oliveira, Afrânio Farias de Melo-Júnior, Elytania Veiga Souza, Luciano Fernandes Souza, V. Royo
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Abstract

El polen de Stryphnodendron polyphyllum (Mart.), típicamente conocido como barbatimão, es un problema para los apicultores con apiarios cerca de la planta, debido a toxicidad del polen para las abejas. El objetivo fue evaluar la actividad antioxidante, antibacteriana, determinar fenoles totales y taninos en el extracto etanólico. La actividad antioxidante se evaluó por método DPPH. La actividad antibacteriana se realizó por método de microdilución. Los taninos totales y fenoles se determinaron por método espectrofotométrico. Tuvimos resultados con actividad inhibitoria y bactericida para Escherichia coli, Pseudomonas aeruginosa, Streptococcus mutans, Streptococcus sobrinus, Streptococcus sanguinis, Streptococcus salivarius y Lactobacillus paracasei. Se determinó EC50 de 7.42mg/mL en la actividad antioxidante. Para compuestos fenólicos y tánicos los resultados comparados con la curva de calibración son 3,12±0,005g/100g de muestra expresados como ácido gálico y 7,3±0,03g/100g de muestra expresados como ácido tánico. Pollen from the Stryphnodendron polyphyllum (Mart.), typically known as barbatimão, is a problem for beekeepers who have apiaries close to the plant, due to pollen toxicity for bees. The objective was to evaluate the antioxidant, antibacterial activity, determine the total phenols and tannins present in the ethanolic extract. Antioxidant activity was evaluated by the DPPH method. Antibacterial activity was performed by the microdilution method.Total tannins and phenols were determined by the spectrophotometric method. We had results with inhibitory and bactericidal activity for Escherichia coli, Pseudomonas aeruginosa, Streptococcus mutans, Streptococcus sobrinus, Streptococcus sanguinis, Streptococcus salivarius and Lactobacillus paracasei. It was determined EC50 of 7.42mg/mL in the antioxidant activity. For phenolic and tannic compounds the results compared to the calibration curve are 3.12±0.005g/100g of sample expressed as gallic acid and 7.3±0.03g/100g of sample expressed as tannic acid.
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Stryphnodedron polyphyllum(Mart.)花粉抗氧化和抗菌潜力的评价
Stryphnodendron polyphyllum(Mart.)的花粉,通常被称为Barbatimão,由于花粉对蜜蜂的毒性,对工厂附近有养蜂人的养蜂人来说是一个问题。目的评估乙醇提取物中的抗氧化、抗菌活性,测定总酚和单宁。用DPPH法测定其抗氧化活性。采用微稀释法进行抗菌活性测定。用分光光度法测定总单宁和酚类物质。结果对大肠杆菌、铜绿假单胞菌、变形链球菌、侄子链球菌、血链球菌、唾液链球菌和副乳杆菌具有抑制和杀菌活性。抗氧化活性测定为7.42mg/ml的EC50。对于酚类和单宁化合物,与校准曲线的比较结果为3.12±0.005g/100g以没食子酸表示的样品和7.3±0.03g/100g以单宁酸表示的样品。来自Stryphnodendron polyphyllum(Mart.)的花粉,通常被称为Barbatimão,对于那些因花粉对蜜蜂的毒性而在工厂附近养蜂的蜂人来说是一个问题。目的评估抗氧化、抗菌活性,测定乙醇提取物中的总酚和单宁。用DPPH法评价其抗氧化活性。采用微稀释法进行抗菌活性。用分光光度法测定总鞣质和酚类物质。我们的结果对大肠杆菌、铜绿假单胞菌、变形链球菌、侄子链球菌、血链球菌、唾液链球菌和副产乳杆菌具有抑制和杀菌活性。抗氧化活性测定为7.42mg/ml的EC50。对于苯酚和鞣酸化合物,与校准曲线相比,结果为3.12±0.005g/100g以加利酸表示的样品和7.3±0.03g/100g以鞣酸表示的样品。
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