Role of nitric oxide in response to high salinity in eelgrass

IF 5.4 Q1 PLANT SCIENCES Current Plant Biology Pub Date : 2023-01-01 DOI:10.1016/j.cpb.2023.100272
Xinfang Lv , Pei Yu , Jie Pan , Xianyan Wang , Yuchun Li
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引用次数: 3

Abstract

Nitric oxide (NO) is an essential molecule for plants, and we have found that NO is mainly synthesized by the nitrate reductase (NR) pathway in eelgrass. To understand the influence of NO on eelgrass, the proline content, the expression of the Na+/H+ antiporter and H+-ATPase, and antioxidant activities were examined under different conditions. The results showed that NO significantly affected these physiological indicators in eelgrass, which played a positive role in eelgrass adaptation to the marine environment. Using RNA-seq, we performed a comprehensive analysis of the genes involved in the NO effects on eelgrass adaptation to high salinity environments. The expression of myosin and glutamine dumper decreased most significantly after inhibiting the NR-dependent NO synthesis. This study shows that the physiological metabolic activities in eelgrass, such as signal transduction process and amino acid metabolism, are closely related to NO under high salinity.

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一氧化氮在鳗草高盐度反应中的作用
一氧化氮(NO)是植物必需的一种分子,我们发现在大叶藻中NO主要通过硝酸还原酶(NR)途径合成。为了解NO对大叶藻的影响,研究了不同条件下大叶藻脯氨酸含量、Na+/H+反转运蛋白和H+- atp酶的表达及抗氧化活性。结果表明,NO显著影响了大叶藻的这些生理指标,对大叶藻适应海洋环境具有积极作用。利用RNA-seq技术,对NO影响大叶藻适应高盐度环境的相关基因进行了全面分析。抑制nr依赖性NO合成后,肌球蛋白和谷氨酰胺转储蛋白的表达下降最为显著。本研究表明,高盐度条件下大叶藻的生理代谢活动,如信号转导过程和氨基酸代谢与NO密切相关。
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来源期刊
Current Plant Biology
Current Plant Biology Agricultural and Biological Sciences-Plant Science
CiteScore
10.90
自引率
1.90%
发文量
32
审稿时长
50 days
期刊介绍: Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.
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