Effect of preventive and simultaneous inoculations of Bacillus amyloliquefaciens (Fukumoto) strains on conidial germination of Botrytis cinerea Pers.:Fr.

A. Hamdache, M. Ezziyyani, A. Lamarti
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引用次数: 6

Abstract

Botrytis cinerea es un patógeno fúngico necrotrófico de varias plantas distribuido en todo el mundo. En el presente estudio se analizó el efecto de nueve aislados bacterianos sobre la germinación de conidios de B. cinerea. Los aislados se identificaron como Bacillus amyloliquefaciens. Para evaluar la eficacia de los aislados, se realizaron confrontaciones duales tanto preventivas como simultáneas con los conidios de B. cinerea. Los resultados mostraron que cinco aislados fueron más eficaces cuando se co-inocularon simultáneamente con el patógeno. Cuatro aislados fueron más eficaces cuando se inocularon previamente cuatro horas antes el patógeno. La cepa denominada B24, mostró una inhibición de (84.04%) en concentraciones muy bajas (3x103 UFC/ml) y la cepa RA9 fue la cepa menos eficaz. Botrytis cinerea is a necrotrophic fungal plant pathogen distributed worldwide. In the current study, the effect of nine bacterial isolates, on germination of B. cinerea conidia were studied. The nine isolates were identified as Bacillus amyloliquefaciens. The efficacy of isolates was tested, at different concentrations, in preventive and simultaneous inoculations with B. cinerea conidia. Results showed that five Bacillus isolates were more effective when co-inoculated simultaneously with the pathogen. Four isolates showed more efficacies when inoculated previously four hours before the pathogen. The isolate denominated B24 was the only that showed an important percent inhibition (84.04 %) at the lower concentration tested (3x103 CFU/ml) and the isolate RA9 was the less effective strain.
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预防和同时接种解淀粉芽孢杆菌(Fukumoto)菌株对灰葡萄孢分生孢子萌发的影响。
灰葡萄孢是一种分布在世界各地的几种植物的坏死真菌病原体。本研究分析了9种细菌分离物对灰霉病菌分生孢子萌发的影响。分离物被鉴定为解淀粉芽孢杆菌。为了评估分离株的有效性,与灰霉病菌的分生孢子进行了预防性和同时性的双重对抗。结果表明,5株菌株与病原体同时接种时效果最好。四个分离株在病原体接种前4小时最有效。名为B24的菌株在非常低的浓度(3x103 cfu/ml)下表现出抑制(84.04%),RA9菌株是最无效的菌株。灰葡萄孢是一种分布在世界各地的坏死真菌植物病原体。在目前的研究中,研究了9种细菌分离物对灰霉病菌分生孢子萌发的影响。这9个菌株被鉴定为解淀粉芽孢杆菌。在不同浓度下,对分离物在预防和同时接种灰霉病菌分生孢子中的有效性进行了测试。结果表明,与病原体同时接种时,五种芽孢杆菌分离株的效果更好。在病原体之前4小时接种疫苗时,四种隔离物显示出更有效的效果。被称为B24的菌株是唯一在最低测试浓度(3x103 CFU/ml)下表现出显著抑制(84.04%)的菌株,而Ra9菌株是最无效的菌株。
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12 weeks
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