Nano-encapsulated tanshinone IIA in PLGA-PEG-COOH inhibits apoptosis and inflammation in cerebral ischemia/reperfusion injury

IF 3.8 4区工程技术 Q2 CHEMISTRY, MULTIDISCIPLINARY Green Processing and Synthesis Pub Date : 2023-01-01 DOI:10.1515/gps-2022-8156

Xin Zhang, Xutong Zhu, Lifa Huang, Zu-peng Chen, Yu-Cai Wang, Yajun Liu, Ruihan Pan, Ling Lv

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引用次数: 1

Abstract

Abstract Tanshinone IIA has a potential therapeutic effect on cerebral ischemia/reperfusion injury (CIRI). In this study, tanshinone IIA was encapsulated in poly(lactic-co-glycolic acid)-block-poly (ethylene glycol)-carboxylic acid (PLGA-PEG-COOH) nanoparticles, and its therapeutic efficacy on CIRI was investigated. Morphology and dynamic light scattering analyses were performed to identify and optimize nano-formulations. A drug release test was conducted using the dialysis method. The cytotoxic effect of tanshinone IIA on human neuroblastoma cells (SH-SY5Y) and brain endothelial capillary cells (hCMEC/D3) was measured using the MTT assay. The protective effect of PLGA-PEG-COOH-encapsulated tanshinone IIA against CIRI was evaluated in oxygen and glucose deprivation/reoxygenation-induced SH-SY5Y/IR cells and middle cerebral artery occlusion (MCAO) rats. Results showed that PLGA-PEG-COOH-encapsulated tanshinone IIA promoted viability and inhibited apoptosis of SH-SY5Y/IR cells (P < 0.01). Moreover, PLGA-PEG-COOH-encapsulated tanshinone IIA facilitated the invasion of SH-SY5Y/IR cells and repressed inflammation in MCAO rats (P < 0.01). Noteworthy, PLGA-PEG-COOH-encapsulated tanshinone IIA combined with angiopep-2 peptide presented a better inhibitory effect on CIRI than tanshinone IIA alone (P < 0.01). Angiopep-2 peptide contributes to traversing blood–brain barrier by recognizing lipoprotein-related protein expressed in the brain capillary endothelial cells. In conclusion, PLGA-PEG-COOH-encapsulated tanshinone IIA plus angiopep-2 peptide holds promising therapeutic potential toward CIRI.

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PLGA-PEG-COOH中的丹参酮IIA纳米胶囊抑制脑缺血/再灌注损伤中的细胞凋亡和炎症反应

摘要丹参酮IIA对脑缺血再灌注损伤(CIRI)具有潜在的治疗作用。本研究将丹参酮IIA包封在聚(乳酸-羟基乙酸)-块聚(乙二醇)-羧酸(PLGA-PEG-COOH)纳米颗粒中，研究其对CIRI的治疗效果。通过形貌和动态光散射分析来确定和优化纳米配方。采用透析法进行药物释放试验。采用MTT法检测丹参酮IIA对人神经母细胞瘤细胞(SH-SY5Y)和脑内皮毛细血管细胞(hCMEC/D3)的细胞毒作用。在缺氧和葡萄糖剥夺/再氧诱导的SH-SY5Y/IR细胞和大脑中动脉闭塞(MCAO)大鼠中，评价plga - peg - cooh包封的丹参酮IIA对CIRI的保护作用。结果表明，plga - peg - cooh包埋的丹参酮IIA可提高SH-SY5Y/IR细胞的生存能力，抑制细胞凋亡(P < 0.01)。此外，plga - peg - cooh包封的丹参酮IIA促进了MCAO大鼠SH-SY5Y/IR细胞的侵袭，抑制了炎症反应(P < 0.01)。值得注意的是，plga - peg - cooh包封的丹参酮IIA联合angiopep-2肽对CIRI的抑制效果优于单用丹参酮IIA (P < 0.01)。Angiopep-2肽通过识别脑毛细血管内皮细胞中表达的脂蛋白相关蛋白，参与穿越血脑屏障。总之，plga - peg - cooh包封的丹参酮IIA +血管内皮素-2肽对CIRI具有良好的治疗潜力。

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公司名称	产品信息	其他信息	采购帮参考价格
上海源叶	丹参酮IIA		￥50.00~￥30459.00

来源期刊

Green Processing and Synthesis CHEMISTRY, MULTIDISCIPLINARY-ENGINEERING, CHEMICAL

CiteScore

6.70

自引率

9.30%

发文量

审稿时长

7 weeks

期刊介绍： Green Processing and Synthesis is a bimonthly, peer-reviewed journal that provides up-to-date research both on fundamental as well as applied aspects of innovative green process development and chemical synthesis, giving an appropriate share to industrial views. The contributions are cutting edge, high-impact, authoritative, and provide both pros and cons of potential technologies. Green Processing and Synthesis provides a platform for scientists and engineers, especially chemists and chemical engineers, but is also open for interdisciplinary research from other areas such as physics, materials science, or catalysis.