{"title":"Assessment of intra- and inter-assay variation in dried blood spot telomere length measurements by qPCR","authors":"Yuki Mizuno, K. Hur, M. Umezaki","doi":"10.1537/ASE.2011301","DOIUrl":null,"url":null,"abstract":"Telomere length has recently gained popularity as a biomarker of aging-related diseases. Dried blood spot (DBS) samples are commonly used to measure telomere length for human biology research, but studies assessing intraand inter-assay variations are scarce. The objective of the present study was to analyze and elucidate the extent of intraand inter-assay variation of DBS telomere length measurement. The DBS telomere length of a male subject was determined by quantitative polymerase chain reaction (qPCR) using four different protocols with two different container types (96-well plates and 8-well tubes) and two different primer sets (tel1b–tel2b and tel1–tel2). The measurement of telomere length with 8-well tubes and the tel1–tel2 primer set demonstrated the lowest intra-assay coefficient of variation (CV) (6.0%) and gave an inter-assay CV of 5.0%. The protocol for 96-well plates typically resulted in high intra-assay CVs (>27%). Assessment of intra-assay variation is essential when DBS telomere length is measured by qPCR.","PeriodicalId":50751,"journal":{"name":"Anthropological Science","volume":" ","pages":""},"PeriodicalIF":1.9000,"publicationDate":"2021-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Anthropological Science","FirstCategoryId":"90","ListUrlMain":"https://doi.org/10.1537/ASE.2011301","RegionNum":4,"RegionCategory":"社会学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"EVOLUTIONARY BIOLOGY","Score":null,"Total":0}
引用次数: 1
Abstract
Telomere length has recently gained popularity as a biomarker of aging-related diseases. Dried blood spot (DBS) samples are commonly used to measure telomere length for human biology research, but studies assessing intraand inter-assay variations are scarce. The objective of the present study was to analyze and elucidate the extent of intraand inter-assay variation of DBS telomere length measurement. The DBS telomere length of a male subject was determined by quantitative polymerase chain reaction (qPCR) using four different protocols with two different container types (96-well plates and 8-well tubes) and two different primer sets (tel1b–tel2b and tel1–tel2). The measurement of telomere length with 8-well tubes and the tel1–tel2 primer set demonstrated the lowest intra-assay coefficient of variation (CV) (6.0%) and gave an inter-assay CV of 5.0%. The protocol for 96-well plates typically resulted in high intra-assay CVs (>27%). Assessment of intra-assay variation is essential when DBS telomere length is measured by qPCR.
期刊介绍：
Anthropological Science (AS) publishes research papers, review articles, brief communications, and material reports in physical anthropology and related disciplines. The scope of AS encompasses all aspects of human and primate evolution and variation. We welcome research papers in molecular and morphological variation and evolution, genetics and population biology, growth and development, biomechanics, anatomy and physiology, ecology and behavioral biology, osteoarcheology and prehistory, and other disciplines relating to the understanding of human evolution and the biology of the human condition.
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