Infeksi Ageratum yellow vein virus pada Gulma Crassocephalum crepidioides di Bengkulu

Abstract

Infection of Ageratum yellow vein virus on Weed Crassocephalum crepidioides in Bengkulu Weeds with symptoms of virus infection were found on three cultivation areas of papaya (Carica papaya) in Bengkulu province, Indonesia. Symptoms on weeds involved yellowing of lamina and vein, yellow mosaic, and leaf curling. This study aimed to identify and detect Begomovirus on weed species, Crassocephalum crepidioides. Virus detection was conducted by polymerase chain reaction method using a pair of universal primers for Begomovirus (SPG1/SPG2). A DNA fragment of 912 bp in size was successfully amplified from C. crepidioides with leaf curl symptom. Analysis using Blastn showed that Begomovirus sample from C. crepidioides has the closest relationship with Ageratum yellow vein virus (AYVV) isolate from Taiwan (DQ866134.1) with nucleotide homology of 99%. This is the first report of AYVV infection in C. crepidioides in Indonesia.