Survival and Plant Injury of Cry2Ab2-susceptible and Resistant Sugarcane Borer (Lepidoptera: Crambidae) on Bt Corn Containing Single or Pyramided Transgenes
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引用次数: 0
Abstract
The sugarcane borer (SCB), Diatraea saccharalis (F.) (Lepidoptera: Crambidae), is a target species of Bacillus thuringiensis (Bt) corn in South America and the mid-southern United States (Huang et al. 2007, Grimi et al. 2018). MON 89034 is a common Bt corn event targeting aboveground lepidopteran pests including SCB (DiFonzo 2020). MON 89034 contains genes for two proteins from Bt: Cry1A.105 and Cry2Ab2. Recently, resistance to Cry1F corn hybrids and crossresistance to the Cry1A.105 protein in MON 89034 have been reported in field populations of SCB in Argentina (Grimi et al. 2018). Thus, it is important to understand the potential for field-evolved resistance to Cry2Ab2 in SCB. Using a F2 screen, we isolated a Cry2Ab2-resistant SCB strain (Cry2A-RR) from a field population collected in Louisiana (Huang et al. 2015). The current study examines the performance of some common transgenic Bt corn traits containing one or multiple Bt genes against the Cry2A-RR. Two SCB strains, Cry2A-RR and a Bt susceptible strain (Bt-SS), were used in this study. The Cry2A-RR strain was developed from a F2 screen with a single two-parental family collected from a non-Bt corn field in Louisiana in 2011. It has been documented to be highly resistant to Cry2Ab2 protein and Cry2Ab2 whole corn plants (Huang et al. 2015). Bt-SS was originally obtained from non-Bt corn fields in Franklin Parish, Louisiana, in 2009. Thereafter, additional field-collected individuals were added into the Bt-SS strain to maintain its genetic diversity. BtSS was confirmed to be susceptible to purified Cry2Ab2, Cry1F, Cry1Ac, Cry1Ab, and Cry1A.105 proteins in diet, and to plant tissue expressing any of these Bt proteins (Ghimire et al. 2011, Wangila et al. 2012, Huang et al. 2015). To ensure a similar genetic background, the original Cry2A-RR described above was backcrossed with Bt-SS and the F2 populations of the backcrossed was reselected on Cry2A-Plant leaf tissue for three more generations as described in the F2 screen
甘蔗螟虫(SCB), Diatraea saccharalis (F.)(鳞翅目:Crambidae),是南美洲和美国中南部苏云金芽孢杆菌(Bacillus thuringiensis, Bt)玉米的目标种(Huang et al. 2007, Grimi et al. 2018)。MON 89034是一种常见的Bt玉米事件,针对地面鳞翅目害虫,包括SCB (DiFonzo 2020)。mon89034含有两种Bt蛋白的基因:Cry1A。105和Cry2Ab2。最近,对Cry1F的抗性玉米杂交种和Cry1A的交叉抗性。据报道,在阿根廷的SCB田间种群中发现了MON 89034中的105蛋白(Grimi et al. 2018)。因此,了解SCB对Cry2Ab2的田间抗性的潜力是很重要的。通过F2筛选,我们从路易斯安那州采集的野外种群中分离出一株抗cry2ab2的SCB菌株(Cry2A-RR) (Huang et al. 2015)。目前的研究考察了一些含有一个或多个Bt基因的常见转基因Bt玉米性状对Cry2A-RR的表现。本研究选用2株SCB, Cry2A-RR和1株Bt敏感株Bt- ss。Cry2A-RR菌株是2011年从路易斯安那州一块非bt玉米田收集的一个双亲家庭的F2筛选中培育出来的。据文献记载,它对Cry2Ab2蛋白和Cry2Ab2全株玉米具有高度抗性(Huang et al. 2015)。Bt-SS最初于2009年从路易斯安那州富兰克林教区的非bt玉米地里获得。随后,将田间收集的个体添加到Bt-SS品系中,以保持其遗传多样性。BtSS被证实对纯化的Cry2Ab2、Cry1F、Cry1Ac、Cry1Ab和Cry1A敏感。以及表达这些Bt蛋白的植物组织(Ghimire et al. 2011, Wangila et al. 2012, Huang et al. 2015)。为了确保相似的遗传背景,将上述原始Cry2A-RR与Bt-SS回交,并将回交的F2群体在Cry2A-Plant叶片组织上重新选择3代,如F2屏幕所示
期刊介绍:
The Journal of Agricultural and Urban Entomology (JAUE) (Journal of Agricultural Entomology, Jan 1984 - Oct 1998 volumes 1-15) is published under the auspices of the South Carolina Entomological Society (SCES). The Journal publishes contributions of original research concerning insects and other arthropods of agricultural and urban importance to include those affecting humans, livestock, poultry, and wildlife. JAUE is particularly dedicated to the publication of articles and notes pertaining to applied entomology, although it will accept suitable contributions of a fundamental nature related to agricultural and urban entomology.