DETERMINATION OF CENTRAL NERVOUS EFFECT AND ANTIDOTAL EFFECT AGAINST CHLORPYRIFOS TOXICITY OF ALPHA-LIPOIC ACID THROUGH PHARMACOLOGICAL AND TOXICOLOGICAL CHALLENGES

Abstract

Toxicity Alpha-Lipoic Acid Abstract Pharmacological and toxicological challenges are among the most important studies in revealing the latent properties of drugs. The goal of this study was to evaluate the effects of alpha-lipoic acid on the central nervous system and chlorpyrifos toxicity in a chick’s model. One-day-old chicks were used, the pharmacological challenge was done through propofol anesthesia and calculate the onset, duration, and recovery time, and while chlorpyrifos was used in the toxicological challenge for the median lethal doses LD 50s were determined using the up-and-down method. Alpha-lipoic acid at 20, 40, and 80 mg/kg caused an inhibition effect on the central nervous system, represented by a significant increase in the anesthesia period when chicks were anesthetized by propofol at 20 mg/kg intraperitoneally. Alpha-lipoic acid caused a significant decrease in the recovery period compared to the control group. Alpha-lipoic acid at 20 and 40 mg/kg increased the value of the median lethal dose of chlorpyrifos by 31 and 62%, respectively. The use of alpha-lipoic acid as preanesthetic has benefits such as decreased recovery time, furthermore Alpha-lipoic acid has a depressant effect in the central nervous system. Alpha-lipoic acid revealed an antidotal effect against chlorpyrifos toxicity.