GENETIC CHARACTERIZATION OF DWARF SNAKEHEAD, Channa gachua (Hamilton, 1822), FROM TWO POPULATIONS BASED ON 16S rRNA GENE

Indonesian Fisheries Research Journal Pub Date : 2021-12-25 DOI:10.15578/ifrj.27.2.2021.109-116

Ragil Pinasti, Warisatul Ilmi, Tuty Ari Suryani

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Abstract

Indonesia is one of the countries with high biodiversity of freshwater fishes. One freshwater fish with a wide distribution in Asia with high economic potency for ornamental fish is dwarf snakehead. However, research on genetic characterization of dwarf snakehead from Indonesia is very limited. Therefore, this research aimed to compare genetic characterization of the dwarf snakehead from rice field irrigation at Donomulyo Village (Malang, East Java) and from Keji River (Magelang, Central Java). This study used a PCR method with universal primers: 16Sar and 16Sbr. The data obtained in this study were then analyzed using DNASTAR, BLAST, Mesquite, MEGA, DnaSP, and NETWORK. The results revealed the genetic distance between dwarf snakehead from rice field irrigation at Donomulyo Village and from Keji River was 1.36%. The divergence of GC content, haplotype number, variables sites, haplotype diversity, and nucleotide diversity in both populations exist. The results of this study are expected to arrange 16S mitochondrial DNA Library of dwarf snakehead from Indonesia which is useful for a reference in the conservation and utilization and management of dwarf snakehead in their habitat.

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基于16S rRNA基因的两个群体矮尾蛇Channa gacchua（Hamilton，1822）的遗传特征

印度尼西亚是淡水鱼类生物多样性较高的国家之一。矮头鱼是亚洲一种分布广泛、经济效益高的观赏淡水鱼。然而，对印尼矮蛇头的遗传特性研究非常有限。因此，本研究旨在比较东爪哇玛琅多诺穆利约村和中爪哇玛格勒克济河稻田灌溉矮生黑鱼的遗传特征。本研究采用PCR方法，通用引物为16Sar和16Sbr。然后使用DNASTAR、BLAST、Mesquite、MEGA、DnaSP和NETWORK分析本研究获得的数据。结果表明，多诺木里约村稻田灌溉矮生黑鱼与科济河矮生黑鱼的遗传距离为1.36%;两种群在GC含量、单倍型数量、变量位点、单倍型多样性、核苷酸多样性等方面存在差异。本研究结果有望建立印度尼西亚矮黑鱼16S线粒体DNA文库，为矮黑鱼栖息地的保护利用和管理提供参考。

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Indonesian Fisheries Research Journal

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12 weeks