Studi Awal Pertumbuhan dan Induksi Mikroalga Haematococcus Pluvialis

Abstract

ABSTRAKMunculnya makanan cepat saji dan polusi udara mendatangkan kerusakan tubuh akibat radikal bebas. Untuk melawan radikal bebas, antioksidan menjadi semakin populer di berbagai kalangan dan salah satunya astaxanthin. Haematococcus pluvialis merupakan sumber astaxanthin alami tertinggi. Tujuan penelitian ini untuk mempelajari pertumbuhan H. Pluvialis. Sebagai variabel dalam penelitian ini adalah (1) konsentrasi inoculum awal yang berbeda (yaitu 10%-v/v dan 20%-v/v) terhadap kepadatan dan jumlah sel; (2) penambahan garam NaCl dan induksi cahaya terhadap rasio karotenoid dan klorofil. Mikroalga H. pluvialis secara fotoautotrof selama sembilan hari. Karotenogenesis diinduksi oleh penambahan NaCl 0,8%-b/v, diikuti oleh induksi di bawah intensitas cahaya tinggi. Kadar klorofil dan total karotenoid dianalisis dengan menggunakan spektrofotometer. Hasil penelitian menunjukkan bahwa pertumbuhan H. pluvialis lebih baik dikulturkan dengan konsentrasi inokulum 10% dan diperoleh jumlah 70 x 105 sel/mL. Penambahan garam NaCl 0,8%-b/v disertai induksi intensitas cahaya tinggi dapat meningkatkan rasio kadar karotenoid terhadap klorofil sebesar 28,9%.Kata kunci:,Haematococcus pluvialis, induksi cahaya, karotenoid, klorofil, mikroalga.ABSTRACTFast food and air pollution lead to the production of free radicals in our body. To fight those, it is needed anti-oxidant. That is the reason why antioxidant become a popular supplement for many people and one of them is astaxanthin. Haematococcus pluvialis is the highest source of natural astaxanthin. The goal of this study is to observe the cell growth of H. pluvialis. The variables used in this research are (1) a different initial inoculum concentrations (i.e. 10%-v/v and 20%-v/v) to the density and number of cells; (2) the addition of salt NaCl and light induction to the ratio of carotenoids to chlorophyll. Microalgae H. pluvialis was cultured in batch mode and photoautotrophic cultivation for nine days. The carotenogenesis was induced by addition of NaCl 0.8%-b/v, followed by induction under high-light intensity. Chlorophyll levels and total carotenoids were analyzed using a spectrophotometer. It was observed that growth of H. pluvialis was preferable cultured with 10% inoculum concentration and obtained 70 x 105 cells/mL. The addition of NaCl 0.8%-b/v salt followed by high light intensity induction could increase the ratio of carotenoids to chlorophyll levels by 28.9%.Keywords: carotenoid, chlorophyll, Haematococcus pluvialis, light induction, microalgae.