Properly interpret metabolic inhibition results to identify primary mercury methylating microbes

IF 11.4 1区 环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES Critical Reviews in Environmental Science and Technology Pub Date : 2023-02-24 DOI:10.1080/10643389.2023.2183072
P. Lei, Ri Yu, Yaqi Kong, S. Bertilsson, M. Tsui, Tao Jiang, Jiating Zhao, Yurong Liu, Rinklebe Joerg, Huan Zhong
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Abstract

Abstract Distinguishing the respective contributions of various microbes to methylmercury (MeHg) production is critical for predicting MeHg bioaccumulation and exposure risk. Metabolic inhibitors have been commonly used to block the activity of specific microbial groups and identify primary Hg methylating microbes. By reviewing literatures and our empirical data, we demonstrate how multiple factors, including (1) the addition of inappropriate amounts of inhibitors, (2) a tendency to overlook microbial syntrophy, and (3) the absence of comprehensive proxy systems of Hg methylation, would impact result interpretation of this approach. We thus suggest that the design of inhibition assays should consider the environmental properties, e.g., background levels of electron acceptors, concentrations of metabolic substrates, and abundances of Hg methylating microbes. We also recommend that inhibitors should be added at multiple concentrations and that observed changes in Hg methylation should be assessed with comprehensive indicators. Revealing the key factors responsible for the improper usage of this method and inadequate interpretation of the results would help optimize inhibition assays for robust predictions of MeHg production in nature. Graphical Abstract
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正确解释代谢抑制结果以鉴定初级汞甲基化微生物
摘要区分各种微生物对甲基汞生产的各自贡献对于预测甲基汞的生物累积和暴露风险至关重要。代谢抑制剂通常用于阻断特定微生物群的活性并鉴定初级汞甲基化微生物。通过回顾文献和我们的经验数据,我们证明了多种因素,包括(1)添加不适当量的抑制剂,(2)忽视微生物共生的倾向,以及(3)缺乏汞甲基化的全面替代系统,将如何影响这种方法的结果解释。因此,我们建议抑制试验的设计应考虑环境特性,例如电子受体的背景水平、代谢底物的浓度和汞甲基化微生物的丰度。我们还建议应添加多种浓度的抑制剂,并用综合指标评估观察到的汞甲基化变化。揭示导致该方法使用不当和对结果解释不充分的关键因素,将有助于优化抑制试验,以可靠预测自然界中甲基汞的产生。图形摘要
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来源期刊
CiteScore
27.30
自引率
1.60%
发文量
64
审稿时长
2 months
期刊介绍: Two of the most pressing global challenges of our era involve understanding and addressing the multitude of environmental problems we face. In order to tackle them effectively, it is essential to devise logical strategies and methods for their control. Critical Reviews in Environmental Science and Technology serves as a valuable international platform for the comprehensive assessment of current knowledge across a wide range of environmental science topics. Environmental science is a field that encompasses the intricate and fluid interactions between various scientific disciplines. These include earth and agricultural sciences, chemistry, biology, medicine, and engineering. Furthermore, new disciplines such as environmental toxicology and risk assessment have emerged in response to the increasing complexity of environmental challenges. The purpose of Critical Reviews in Environmental Science and Technology is to provide a space for critical analysis and evaluation of existing knowledge in environmental science. By doing so, it encourages the advancement of our understanding and the development of effective solutions. This journal plays a crucial role in fostering international cooperation and collaboration in addressing the pressing environmental issues of our time.
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