{"title":"CRISPR_Cas systems for fungal research","authors":"Jean-Paul Ouedraogo, Adrian Tsang","doi":"10.1016/j.fbr.2020.10.002","DOIUrl":null,"url":null,"abstract":"<div><p>Genome-editing CRISPR-Cas systems, using Cas9 and Cas12a endonucleases, have improved our ability to precisely edit genomes and control gene expression. We summarize here the knowledge gained from using CRISPR-Cas9 and CRISPR-Cas12a in fungal research. Also discussed are strategies developed for limiting the occurrences of off-target mutations caused by CRISPR-Cas genome editing.</p></div>","PeriodicalId":12563,"journal":{"name":"Fungal Biology Reviews","volume":"34 4","pages":"Pages 189-201"},"PeriodicalIF":5.7000,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.fbr.2020.10.002","citationCount":"22","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fungal Biology Reviews","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1749461320300403","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MYCOLOGY","Score":null,"Total":0}
引用次数: 22
Abstract
Genome-editing CRISPR-Cas systems, using Cas9 and Cas12a endonucleases, have improved our ability to precisely edit genomes and control gene expression. We summarize here the knowledge gained from using CRISPR-Cas9 and CRISPR-Cas12a in fungal research. Also discussed are strategies developed for limiting the occurrences of off-target mutations caused by CRISPR-Cas genome editing.
期刊介绍：
Fungal Biology Reviews is an international reviews journal, owned by the British Mycological Society. Its objective is to provide a forum for high quality review articles within fungal biology. It covers all fields of fungal biology, whether fundamental or applied, including fungal diversity, ecology, evolution, physiology and ecophysiology, biochemistry, genetics and molecular biology, cell biology, interactions (symbiosis, pathogenesis etc), environmental aspects, biotechnology and taxonomy. It considers aspects of all organisms historically or recently recognized as fungi, including lichen-fungi, microsporidia, oomycetes, slime moulds, stramenopiles, and yeasts.
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