{"title":"Flow-Cytometry-Based Method to Detect Escherichia coli and Shigella Spp. Using 16S rRNA-Based Probe","authors":"Yong Xue, Jon G. Wilkes, Ted J. Moskal, Anna J. Williams, Willie M. Cooper, Dan A. Buzatu","doi":"10.1002/cptx.14","DOIUrl":null,"url":null,"abstract":"<p>Detection of microbial contamination in foods before they go on to the market can help prevent the occurrence of foodborne illness outbreaks. Current methods for the detection of <i>Escherichia coli</i> are limited by time-consuming procedures, which include multiple culture incubation steps, and require several days to get results. This unit describes the development of an improved rapid flow-cytometry-based detection method that has greater sensitivity and specificity. This method requires less time-to-results (TTR) and can detect a small number of <i>E. coli</i> in the presence of large numbers of other bacteria. Clear step-by-step protocols for cell concentration determination, sample preparation, and flow cytometric analysis are provided. © 2017 by John Wiley & Sons, Inc.</p>","PeriodicalId":72743,"journal":{"name":"Current protocols in toxicology","volume":"71 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2017-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cptx.14","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current protocols in toxicology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cptx.14","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
Detection of microbial contamination in foods before they go on to the market can help prevent the occurrence of foodborne illness outbreaks. Current methods for the detection of Escherichia coli are limited by time-consuming procedures, which include multiple culture incubation steps, and require several days to get results. This unit describes the development of an improved rapid flow-cytometry-based detection method that has greater sensitivity and specificity. This method requires less time-to-results (TTR) and can detect a small number of E. coli in the presence of large numbers of other bacteria. Clear step-by-step protocols for cell concentration determination, sample preparation, and flow cytometric analysis are provided. © 2017 by John Wiley & Sons, Inc.
基于流式细胞术的16S rrna探针检测大肠杆菌和志贺氏菌的方法
在食品进入市场之前对其进行微生物污染检测有助于预防食源性疾病暴发的发生。目前检测大肠杆菌的方法受限于耗时的程序,其中包括多个培养孵育步骤,并且需要几天才能得到结果。本单元描述了一种改进的基于流式细胞术的快速检测方法的发展,该方法具有更高的灵敏度和特异性。这种方法需要较少的时间到结果(TTR),并且可以在存在大量其他细菌的情况下检测到少量大肠杆菌。明确一步一步的方案,细胞浓度测定,样品制备和流式细胞术分析提供。©2017 by John Wiley &儿子,Inc。
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