Evaluation of blood samples collected by dried blood spots (DBS) method for hepatitis B virus DNA quantitation and its stability under real life conditions

IF 1.6 Q4 INFECTIOUS DISEASES Journal of clinical virology plus Pub Date : 2022-11-01 DOI:10.1016/j.jcvp.2022.100111

Rahul Garg, Krithiga Ramachandran, S. Jayashree, Reshu Agarwal, Ekta Gupta

{"title":"Evaluation of blood samples collected by dried blood spots (DBS) method for hepatitis B virus DNA quantitation and its stability under real life conditions","authors":"Rahul Garg, Krithiga Ramachandran, S. Jayashree, Reshu Agarwal, Ekta Gupta","doi":"10.1016/j.jcvp.2022.100111","DOIUrl":null,"url":null,"abstract":"<div><p>We evaluated the diagnostic performance of dried blood spots (DBS) compared to plasma for detection and quantification of HBV-DNA under real-life conditions. Blood specimens from 100 known cases of chronic hepatitis B (CHB) requested for quantitative HBV-DNA were included. In a subset of 20 patients, three sets of DBS cards were prepared, one kept at -80°C, the other two kept at room temperature for 7 and 14 days, respectively. DBS method demonstrated sensitivity, specificity, and positive predictive value of 95.3%, 100%, and 100%, respectively in comparison to plasma. The mean HBV-DNA load in plasma was 4.8 log10 IU/ml while in DBS was 4.3 log10 IU/ml with a strong correlation (R<sup>2</sup>=0.9087). No significant change in viral load was observed at room temperature for up to 14 days. This study suggests that DBS for HBV viral-load quantitation is a good alternative to plasma as it is stable during storage at room temperature and therefore allows easy handling, storage, and transport of specimens in resource-limited settings.</p></div>","PeriodicalId":73673,"journal":{"name":"Journal of clinical virology plus","volume":"2 4","pages":"Article 100111"},"PeriodicalIF":1.6000,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667038022000503/pdfft?md5=c6f1e3cde94c7563efe14a552e824ac0&pid=1-s2.0-S2667038022000503-main.pdf","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of clinical virology plus","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2667038022000503","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}

引用次数: 2

Abstract

We evaluated the diagnostic performance of dried blood spots (DBS) compared to plasma for detection and quantification of HBV-DNA under real-life conditions. Blood specimens from 100 known cases of chronic hepatitis B (CHB) requested for quantitative HBV-DNA were included. In a subset of 20 patients, three sets of DBS cards were prepared, one kept at -80°C, the other two kept at room temperature for 7 and 14 days, respectively. DBS method demonstrated sensitivity, specificity, and positive predictive value of 95.3%, 100%, and 100%, respectively in comparison to plasma. The mean HBV-DNA load in plasma was 4.8 log10 IU/ml while in DBS was 4.3 log10 IU/ml with a strong correlation (R²=0.9087). No significant change in viral load was observed at room temperature for up to 14 days. This study suggests that DBS for HBV viral-load quantitation is a good alternative to plasma as it is stable during storage at room temperature and therefore allows easy handling, storage, and transport of specimens in resource-limited settings.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

血斑干法测定乙型肝炎病毒DNA的评价及其在实际条件下的稳定性。

我们评估了干燥血斑(DBS)与血浆在现实条件下检测和定量HBV-DNA的诊断性能。来自100例已知慢性乙型肝炎(CHB)病例的血液标本被要求进行HBV-DNA定量分析。在20例患者中，准备了三套DBS卡，一套保存在-80°C，另两套保存在室温下，分别保存7天和14天。与血浆相比，DBS方法的敏感性、特异性和阳性预测值分别为95.3%、100%和100%。血浆中HBV-DNA平均负荷为4.8 log10 IU/ml, DBS中为4.3 log10 IU/ml，相关性较强(R2=0.9087)。在室温下，病毒载量未见显著变化长达14天。该研究表明，DBS用于HBV病毒载量定量是血浆的一种很好的替代方法，因为它在室温下储存时稳定，因此在资源有限的情况下易于处理、储存和运输标本。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊