Interactions of arene ruthenium(II) complexes [η6-(C6H6)Ru(pprip)Cl]+ and [η6-(C6H6)Ru(H2iiP)Cl]+ with RNA triplex poly(U)•poly(A)*poly(U)

IF 2.7 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY JBIC Journal of Biological Inorganic Chemistry Pub Date : 2023-07-21 DOI:10.1007/s00775-023-02008-y
Feng Yuan, Xiaohua Liu, Juan Li, Lifeng Tan
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引用次数: 1

Abstract

Two arene ruthenium(II) complexes [η6-(C6H6)Ru(pprip)Cl]PF6 (Ru1; pprip = 2-(3-phenyl-1H-pyrazol-4-yl)-imidazolo[4,5-f][1,10]phenanthroline) and [η6-(C6H6)Ru(H2iiP)Cl]PF6 (Ru2; H2iiP = 2-(indole-3-yl)-imidazolo[4,5-f][1,10]phenanthroline) have been synthesized and characterized in this work. Binding properties of Ru1 and Ru2 with the triplex RNA poly(U)•poly(A)*poly(U) were investigated by spectrophotometry and spectrofluorometry as well as viscosimetry. Analysis of spectroscopic titrations and viscosity measurements show that the two complexes bind with the triplex through intercalation, while the binding affinity for Ru2 toward the triplex is stronger than that for Ru1. Melting experiments indicate that the stabilizing effects of Ru1 and Ru2 toward the triplex differ from each other. Under the conditions used herein, Ru1 only stabilizes the Hoogsteen base-paired strand (third strand) without affecting stabilization of the Watson–Crick base-paired strand (the template duplex) of the triplex, while Ru2 stabilizes both the template duplex and the third strand. Although the two complexes prefer to stabilizing the third strand rather than the template duplex, the third-strand stabilization effect of Ru2 is stronger than that of Ru1. The obtained results of this work reveal that the planarity of the intercalative ligands plays an important role in the triplex stabilization by arene Ru(II) complexes.

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芳烃钌(II)配合物[η6-(C6H6)Ru(pprip)Cl]+和[η6-(C6H6)Ru(H2iiP)Cl]+与RNA三元聚(U)•聚(A)*聚(U)的相互作用
二芳烃钌(II)配合物[η6-(C6H6)Ru(pprip)Cl]PF6 (Ru1;pprip = 2-(3-苯基- 1h -吡唑-4-基)-咪唑啉[4,5-f][1,10]菲罗啉]和[η6-(C6H6)Ru(H2iiP)Cl]PF6 (Ru2;H2iiP = 2-(吲哚-3-基)-咪唑[4,5-f][1,10]菲罗啉]的合成和表征。采用分光光度法、荧光光谱法和粘度法研究了Ru1和Ru2与三重RNA poly(U)•poly(A)*poly(U)的结合特性。光谱滴定分析和粘度测量表明,这两个配合物通过插层与三联体结合,而Ru2对三联体的结合亲和力比Ru1强。熔融实验表明,Ru1和Ru2对三相体的稳定作用不同。在本文使用的条件下,Ru1仅稳定Hoogsteen碱基配对链(第三链),而不影响三联体中Watson-Crick碱基配对链(模板双链)的稳定,而Ru2稳定模板双链和第三链。虽然这两种配合物更倾向于稳定第三链而不是模板双链,但Ru2的第三链稳定作用强于Ru1。研究结果表明,嵌入配体的平面性对芳烃Ru(II)配合物的三络合物稳定起着重要的作用。图形抽象
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来源期刊
JBIC Journal of Biological Inorganic Chemistry
JBIC Journal of Biological Inorganic Chemistry 化学-生化与分子生物学
CiteScore
5.90
自引率
3.30%
发文量
49
审稿时长
3 months
期刊介绍: Biological inorganic chemistry is a growing field of science that embraces the principles of biology and inorganic chemistry and impacts other fields ranging from medicine to the environment. JBIC (Journal of Biological Inorganic Chemistry) seeks to promote this field internationally. The Journal is primarily concerned with advances in understanding the role of metal ions within a biological matrix—be it a protein, DNA/RNA, or a cell, as well as appropriate model studies. Manuscripts describing high-quality original research on the above topics in English are invited for submission to this Journal. The Journal publishes original articles, minireviews, and commentaries on debated issues.
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