Comparative Karyomorphological Analysis of In vivo and In vitro Grown Plants of Plumbago zeylanica: An Important Medicinal Plant

Abstract

The present report describes the comparative cytological analysis of in vivo and in vitro grown plant species of Plumbago zeylanica. Somatic chromosome number of in vivo and micro-propagated plants was confirmed to be 2n=28. Individual chromosome length of mother plant and micro-propagated plant was ranged from 2.26 to 5.54 µm and 2.26 to 5.46 µm, respectively. The total length of the haploid complement of in vivo and in vitro grown plants was 49.35 and 52.98 µm. The total form percent (TF%) of mother and in vitro grown plants was 45.11% and 45.58%, respectively and according to Stebbins classification (1971) both plants karyotype was fell into 2B symmetric type. The centromeric formula was for in vivo and in vitro grown plants were 3sm + 11m.