Optimization of Nano Science Parameters for Extracting Phenolic Compounds from Ficus religiosa by Three-Phase Partitioning (TPP) Method

Abstract

The phenolic compounds are generally found in different parts of the plants such as leaves, barks, seeds, fruits, and so on. These compounds show numerous nanoscience-based bioactive properties including antioxidant characteristics. The phenolic compounds obtained from beneficial herbs and dietary plants include total phenolic compounds, flavonoids, and tannins. In recent times, nanoscience has proved to be extensively reliable in extracting bioactive components. Additionally, nanomaterials have made a considerable contribution in the development of methodical techniques to retain superiority in processing foods and medicines. Of late, extracting nano-bioactive composites from natural resources have gained high interest as these composites are used in manufacturing a wide range of products such as foods, paints, and pharmaceuticals. Therefore, there is a requirement to separate natural products and identify new nano-bioactive compounds that have the potential to improve the importance of logical and developed techniques. This research is focused on optimizing the experimental parameters to extract the phenolic compounds from Ficus religiosa (banyan leaves) by the Three-Phase Partitioning (TPP) method. TPP is an advanced method that has been used for the extraction, concentration, and purification of various nano-based bioactive compounds and enzymes. During the investigation, various experimental parameters have been studied to obtain the maximum concentration of phenolic compounds from the dried powder of the Ficus religiosa leaves. The optimised results were found as ammonium sulphate by 30%, solute to the solvent ratio in 1:20 (v/v), and slurry to t-butanol ratio in 1:1 proportions.