Natural product nanofibers derived from Trichoderma hamatum K01 to control citrus anthracnose caused by Colletotrichum gloeosporioides

IF 1.8 Q2 AGRICULTURE, MULTIDISCIPLINARY Open Agriculture Pub Date : 2023-01-01 DOI:10.1515/opag-2022-0193

Pheaktra Phal, K. Soytong, S. Poeaim

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引用次数: 1

Abstract

Abstract The antifungal activity of Trichoderma hamatum K01 to control Colletotrichum gloeosporioides C01 was recorded. Pathogenicity of C. gloeosporioides C01 on citrus leaves was confirmed by the Koch postulate. T. hamatum K01 inhibited the colony growth and conidia production of C. gloeosporioides C01 by 70.55 and 79.07%, respectively. Crude extracts from T. hamatum K01 expressed an antifungal activity against C. gloeosporioides C01. The crude TK01-MeOH showed the most potent inhibitory effect on the colony growth and conidia production at the ED50 values of 273 and 355 ppm, respectively. Nanofiber TK01M gave the highest inhibition on the colony growth and conidia production at the ED50 values of 13 and 3 ppm, respectively. Control mechanism was related to the major pyrone metabolite group (6-pentyl-2H-pyran-2-one), organic compounds (benzoic acid, hexadecane, tetracosane), fatty acids (palmitic acid, linoleic acid, tetradecanoic acid, pentadecanoic acid, hexadecenoic acid, ethyl ester, linoleic acid ethyl ester, and ethyl oleate), and sorbicillin from GC-MS analysis, which were produced by T. hamatum K01. It is reported for the first time to isolate secondary metabolite pyrone (6-pentyl-2H-pyran-2-one) from T. hamatum K01, and the first report of nanofibers constructed from T. hamatum K01 against C. gloeosporioides.

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红木木霉K01天然产物纳米纤维防治柑橘炭疽病

摘要报道了木霉K01对炭疽杆菌C01的抗真菌活性。通过Koch假设证实了粘孢子虫C01对柑桔叶片的致病性。哈氏T.hamatum K01对球孢C.gloeosporioides C01的菌落生长和分生孢子的产生分别抑制了70.55%和79.07%。来自T.hamatum K01的粗提取物表达了对C.gloeosporioides C01的抗真菌活性。在ED50值为273和355时，粗TK01 MeOH对菌落生长和分生孢子产生的抑制作用最强 ppm。纳米纤维TK01M在ED50值为13和3时对菌落生长和分生孢子产生的抑制作用最高 ppm。通过GC-MS分析，控制机制与主要的吡喃酮代谢产物组（6-戊基-2H-吡喃-2-酮）、有机化合物（苯甲酸、十六烷、四烷）、脂肪酸（棕榈酸、亚油酸、十四烷酸、十五烷酸、十六碳烯酸、乙酯、亚油酸乙酯和油酸乙酯）和山梨酸有关。首次报道了从T.hamatum K01中分离出次级代谢产物吡喃酮（6-戊基-2H-吡喃-2-酮），并首次报道了由T.hamatumK01构建的纳米纤维对抗球孢菌。

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来源期刊

Open Agriculture AGRICULTURE, MULTIDISCIPLINARY-

CiteScore

3.80

自引率

4.30%

发文量

审稿时长

9 weeks

期刊介绍： Open Agriculture is an open access journal that publishes original articles reflecting the latest achievements on agro-ecology, soil science, plant science, horticulture, forestry, wood technology, zootechnics and veterinary medicine, entomology, aquaculture, hydrology, food science, agricultural economics, agricultural engineering, climate-based agriculture, amelioration, social sciences in agriculuture, smart farming technologies, farm management.