Yang Shen, Xiaolong Shen, Hao Zhang, Xin Li, Tengda Shang, Yuancong Zhao, Jin Wang, Nan Huang
{"title":"Improved corrosion resistance and biocompatibility of biomedical magnesium alloy with polypeptide TK14 functionalised hydrophobic coating","authors":"Yang Shen, Xiaolong Shen, Hao Zhang, Xin Li, Tengda Shang, Yuancong Zhao, Jin Wang, Nan Huang","doi":"10.1049/bsb2.12011","DOIUrl":null,"url":null,"abstract":"<p>Magnesium (Mg) and its alloys can be used as biomedical materials because of their excellent mechanical properties and biocompatibility. However, the rapid degradation rate of Mg-based materials limits their application in biodegradable intravascular stents. To overcome this issue, we constructed a hydrophobic coating on magnesium. After pre-treatments with alkali and a silane coupling agent of pure magnesium, 4,4’-diphenylmethane-diisocyanate (MDI) and amino-terminated polydimethylsiloxane (H<sub>2</sub>N–PDMS–NH<sub>2</sub>) were stepwise deposited on the surface, forming an amino-containing hydrophobic coating (–(M/P)<sub>3</sub>) to enhance the corrosion resistance. Furthermore, polypeptide TK14 was immobilised on the hydrophobic coating to promote vascular endothelial cell adhesion and proliferation. The electrochemical results revealed that the self-corrosion current density (<i>i</i><sub>corr</sub>) of –(M/P)<sub>3</sub> decreased by approximately 4.5 orders of magnitude compared with that of pure Mg. After TK14 immobilisation, the number of endothelial cells adhering to the surface of –(M/P)<sub>3</sub>–T increased significantly. Although the corrosion resistance of –(M/P)<sub>3</sub>–T was slightly reduced, the subcutaneous implantation inflammatory response of the surrounding tissues was lower, showing suitable biocompatibility. Therefore, the polypeptide TK14 functionalised hydrophobic coating may be a promising candidate material for the interface of magnesium-based cardiovascular implants.</p>","PeriodicalId":52235,"journal":{"name":"Biosurface and Biotribology","volume":"7 1","pages":"12-22"},"PeriodicalIF":1.6000,"publicationDate":"2021-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ietresearch.onlinelibrary.wiley.com/doi/epdf/10.1049/bsb2.12011","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biosurface and Biotribology","FirstCategoryId":"1087","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1049/bsb2.12011","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}
引用次数: 1
Abstract
Magnesium (Mg) and its alloys can be used as biomedical materials because of their excellent mechanical properties and biocompatibility. However, the rapid degradation rate of Mg-based materials limits their application in biodegradable intravascular stents. To overcome this issue, we constructed a hydrophobic coating on magnesium. After pre-treatments with alkali and a silane coupling agent of pure magnesium, 4,4’-diphenylmethane-diisocyanate (MDI) and amino-terminated polydimethylsiloxane (H2N–PDMS–NH2) were stepwise deposited on the surface, forming an amino-containing hydrophobic coating (–(M/P)3) to enhance the corrosion resistance. Furthermore, polypeptide TK14 was immobilised on the hydrophobic coating to promote vascular endothelial cell adhesion and proliferation. The electrochemical results revealed that the self-corrosion current density (icorr) of –(M/P)3 decreased by approximately 4.5 orders of magnitude compared with that of pure Mg. After TK14 immobilisation, the number of endothelial cells adhering to the surface of –(M/P)3–T increased significantly. Although the corrosion resistance of –(M/P)3–T was slightly reduced, the subcutaneous implantation inflammatory response of the surrounding tissues was lower, showing suitable biocompatibility. Therefore, the polypeptide TK14 functionalised hydrophobic coating may be a promising candidate material for the interface of magnesium-based cardiovascular implants.