In vivo construction of lymphoid node by implantation of adipose-derived stromal cells with hydroxypropyl methyl cellulose hydrogel in BALB/c nude mice

IF 1.6 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Organogenesis Pub Date : 2019-07-03 DOI:10.1080/15476278.2019.1656994

Jing Zhang, Yuqiao Xu, Tao Liu, Jie Min, Yu Ma, Yongli Song, Jianrong Lu, Wen-juan Mi, Yingmei Wang, Hang Li, Wangzhou Li, Da-Qing Zhao

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引用次数: 3

Abstract

ABSTRACT Adipose-derived stromal cells have multilineage potential to differentiate into several specialized tissue types. Herein, we investigated whether ADSCs could differentiate into lymphoid node in vivo. Human ADSCs from routine liposuction were cultured in differentiation medium and were supplemented with transforming growth factor β1 (TGF)-β1 and basic fibroblast growth factor (bFGF). The induced hADSCs mixed with 13% (w/v) hydroxypropyl methylcellulose (HPMC) were injected into BALB/c nude mice subcutaneously. Eight weeks later, nodules were found under the injected sites. Histology, immunohistochemistry, and species identification analysis confirmed that the nodules were lymphoid nodes that were derived from the injected hADSCs. Our experiment demonstrated that the hADSCs could differentiate into lymphocyte-like cells and form lymphoid nodes in vivo. TGF-β1 and bFGF might play important roles in the differentiation of hADSCs into lymphocyte-like cells. Our study might present an alternative approach for engineering immune organs and thus offer potential treatment for immunodeficiency diseases.

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羟丙基甲基纤维素水凝胶植入脂肪基质细胞在BALB/c裸鼠体内构建淋巴结

脂肪来源的基质细胞具有多谱系分化成几种特化组织类型的潜力。在此，我们研究了ADSCs是否能在体内向淋巴结分化。将常规抽脂后的人ADSCs在分化培养基中培养，并添加转化生长因子β1 (TGF)-β1和碱性成纤维细胞生长因子(bFGF)。将诱导的hscs与13% (w/v)羟丙基甲基纤维素(HPMC)混合后皮下注射BALB/c裸鼠。8周后，在注射部位发现结节。组织学、免疫组织化学和物种鉴定分析证实结节是来源于注射的hascs的淋巴结。我们的实验表明，hscs在体内可以分化为淋巴细胞样细胞并形成淋巴结。TGF-β1和bFGF可能在hADSCs向淋巴细胞样细胞分化过程中发挥重要作用。我们的研究可能为工程免疫器官提供另一种方法，从而为免疫缺陷疾病提供潜在的治疗方法。

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来源期刊

Organogenesis BIOCHEMISTRY & MOLECULAR BIOLOGY-DEVELOPMENTAL BIOLOGY

CiteScore

4.10

自引率

4.30%

发文量

审稿时长

>12 weeks

期刊介绍： Organogenesis is a peer-reviewed journal, available in print and online, that publishes significant advances on all aspects of organ development. The journal covers organogenesis in all multi-cellular organisms and also includes research into tissue engineering, artificial organs and organ substitutes. The overriding criteria for publication in Organogenesis are originality, scientific merit and general interest. The audience of the journal consists primarily of researchers and advanced students of anatomy, developmental biology and tissue engineering. The emphasis of the journal is on experimental papers (full-length and brief communications), but it will also publish reviews, hypotheses and commentaries. The Editors encourage the submission of addenda, which are essentially auto-commentaries on significant research recently published elsewhere with additional insights, new interpretations or speculations on a relevant topic. If you have interesting data or an original hypothesis about organ development or artificial organs, please send a pre-submission inquiry to the Editor-in-Chief. You will normally receive a reply within days. All manuscripts will be subjected to peer review, and accepted manuscripts will be posted to the electronic site of the journal immediately and will appear in print at the earliest opportunity thereafter.