Evaluation of liposomal hydrocolloidal NPs loaded by tea tree oil as antifungal agent in vitro and in vivo investigations: Preclinical studies

IF 4.6 Q1 CHEMISTRY, APPLIED Food Hydrocolloids for Health Pub Date : 2023-04-30 DOI:10.1016/j.fhfh.2023.100136
Ahmed M. Abd El- Salam , Amin Tahoun , Nemany A.N. Hanafy
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引用次数: 2

Abstract

Fungi have a great ability and a wide variety of mechanisms to endure the toxicity of current antifungal agents. Researchers are working to find new therapeutic agents to combat the resistance ability of fungi. Almost all commercial antifungal agents have a wide variety of side effects on human health. This study aims to introduce tea tree oil nanoparticles as antifungal delivery termed TNSAD, which contains hybrid tea tree oil attached chitosan in a liposomal formulation, and assess its antifungal activity using in vitro and in vivo infection models. Tea tree oil was first coated by chitosan, then inserted inside liposomal bilayers, and finally functionalized by a layer of chitosan, forming the TNSAD. The antifungal activity was evaluated against four different invasive, opportunistic, and zoonotic fungal pathogens (Aspergillus flavus, Aspergillus fumigatus, Microsporum gypsum, and Fusarium oxysporum). The cytotoxicity of TNSAD was then tested against the HEp-2 cell line. Finally, the antifungal activity against Aspergillus fumigatus and Microsporum gypsum was assessed in vivo in a rat model. The in vitro results confirm the potency of TNSAD against fungi and its safety at a concentration of ≤ 5 mg/ml. In vivo results revealed that fungal cells were destroyed within the tissue when used systematically. we have described here a natural remarkable design that represents a potential antifungal agent and provided evidence for its efficiency and safety, which makes it a promising antifungal agent for the treatment of systemic and topical fungal infections.

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茶树油负载的脂质体水胶体NPs作为体外和体内抗真菌剂的评价:临床前研究
真菌具有强大的能力和多种机制来承受当前抗真菌药物的毒性。研究人员正在努力寻找新的治疗药物来对抗真菌的抗药性。几乎所有的商业抗真菌剂对人体健康都有各种各样的副作用。本研究旨在引入茶树油纳米颗粒作为抗真菌递送剂,即TNSAD,该纳米颗粒在脂质体中含有混合茶树油和壳聚糖,并通过体外和体内感染模型评估其抗真菌活性。茶树油先被壳聚糖包被,然后插入到脂质体双层中,最后被一层壳聚糖功能化,形成TNSAD。对四种不同的侵袭性、机会性和人畜共患性真菌病原体(黄曲霉、烟曲霉、石膏小孢子菌和尖孢镰刀菌)的抗真菌活性进行了评估。然后检测TNSAD对HEp-2细胞株的细胞毒性。最后,在大鼠体内模型中评价其对烟曲霉和小孢子菌的抗真菌活性。体外实验结果证实了TNSAD对真菌的抑菌作用和浓度≤5 mg/ml时的安全性。体内实验结果显示,当系统使用时,真菌细胞在组织内被破坏。我们在这里描述了一种天然的显著设计,它代表了一种潜在的抗真菌药物,并为其有效性和安全性提供了证据,这使得它成为治疗全身和局部真菌感染的有前途的抗真菌药物。
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来源期刊
CiteScore
4.50
自引率
0.00%
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0
审稿时长
61 days
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