Validation of molecular markers linked to flowering time genes in chickpea (Cicer arietinum L.)

IF 1 4区生物学 Q3 PLANT SCIENCES Indian Journal of Genetics and Plant Breeding Pub Date : 2022-02-25 DOI:10.31742/ijgpb.82.1.14

Aswini Nunavath, V. Hegde, C. Bharadwaj, S. Tripathi, Rajendra Kumar, P. Jain, R. Singh, Supriya Sachdeva

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Abstract

The objective of the study was to validate molecular markers linked to genomic regions controlling flowering time in a set of Recombinant Inbred Lines (RILs) population derived from Pusa 362 (Late) x BGD 132 (Early) and 21 diverse genotypes differing in time of flowering in chickpea (Cicer arietinum L.). The study of the polymorphic survey between parents using 27 linked markers of chickpea and Arabidopsis found 8 of them polymorphic. Bulked Segregant Analysis identified TA64 and TA142 as putatively linked to efl3 locus governing early flowering in chickpea that can be utilized for marker assisted selection. The PCR amplification of 6 polymorphic markers in germplasm showed the presence of one or more alleles in many of them (up to 3), indicating the complex nature of flowering time in chickpea. The amplification pattern of CaEfl3a-F1 marker linked to Efl3 early flowering allele of Arabidopsis in 21 chickpea germplasm indicated the presence of some conserved genomic sequences controlling flowering time in the two species.

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鹰嘴豆开花时间基因分子标记的验证

本研究的目的是验证一组来自Pusa 362（Late）x BGD 132（Early）和21种不同基因型鹰嘴豆（Cicer arietinum L.）的重组自交系（RILs）群体中与控制开花时间的基因组区域相连的分子标记拟南芥发现其中8个具有多态性。Bulked Segregant分析确定TA64和TA142被认为与调控鹰嘴豆早期开花的efl3基因座相连，可用于标记辅助选择。对种质中6个多态性标记的PCR扩增显示，其中许多（多达3个）标记中存在一个或多个等位基因，表明鹰嘴豆开花时间的复杂性。在21份鹰嘴豆种质中，与拟南芥Efl3早花等位基因连接的CaEfl3a-F1标记的扩增模式表明，在这两个物种中存在一些控制开花时间的保守基因组序列。

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来源期刊

Indian Journal of Genetics and Plant Breeding PLANT SCIENCES-

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Advance the cause of genetics and plant breeding and to encourage and promote study and research in these disciplines in the service of agriculture; to disseminate the knowledge of genetics and plant breeding; provide facilities for association and conference among students of genetics and plant breeding and for encouragement of close relationship between them and those in the related sciences; advocate policies in the interest of the nation in the field of genetics and plant breeding, and facilitate international cooperation in the field of genetics and plant breeding.