Entomopathogenic fungi on the control of Sphenophorus levis in sugarcane crop

Fernando Belezini Vinha, Luis Arnaldo de Assis Delfanti, V. Pagliarani, Vitor S Ferreira, A. Pinto
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Abstract

Brazil is the largest producer of sugarcane crop in the world,but its production can be highly affected by the pest Sphenophorus levis. The entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana can be used for the control of this pest, but are not always effective in the field, lacking information on application methods, doses and formulations. We evaluate the efficacy of M. anisopliae IBCB 425 and B.bassiana IBCB 66 in controlling S. levis and other soil pests at sugarcane crop. The bioassay was conducted in a split plot design, where each of the 4 treatments were repeated 5 times and distributed over an area of 5.000 m 2 . The treatments for both fungi consisted of: Wettable powder (WP) formulation 225 g conidia ha -1 , WP formulation 450 g conidia ha -1 , granule (GR) formulation 10 Kg ha -1 and Control (no fungal application). WP fungi formulations were applied to the ratoon with a tractor using a ratoon cutter and an pesticide applicator. The GR fungi formulation was manually applied on the ratoons. The evaluations were performed after 21, 60 and 100 days of fungi application. B. Bassiana fungus reduced the pest population but did not differ from the control, however for M. Anisopliae fungus there were significant differences between treatments on damaged stems. The fungus M. anisopliae was effective in controlling S. levis larvae and adults in the field and WP formulation had faster action than the GR, the dose of 225 g conidia ha -1 was enought to reduce S. levis population.
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昆虫病原真菌防治甘蔗褐飞虱的研究
巴西是世界上最大的甘蔗生产国,但它的产量可能受到甘蔗害虫Sphenophorus levis的严重影响。昆虫病原真菌绿僵菌和球孢白僵菌可用于防治这种害虫,但由于缺乏有关应用方法、剂量和配方的信息,在田间并不总是有效的。研究了金龟子僵菌IBCB 425和球孢僵菌IBCB 66对甘蔗土壤害虫李氏僵菌和其他土壤害虫的防治效果。生物测定采用裂区设计,4个处理各重复5次,分布在5000平方米的面积上。两种真菌的处理均为:湿性粉剂225 g分生孢子ha -1,湿性粉剂450 g分生孢子ha -1,颗粒剂10 Kg ha -1和对照(不施用真菌)。用拖拉机、割草机和农药喷洒器将WP真菌制剂施用于水稻上。人工将GR真菌制剂涂在菌丝上。分别在施用真菌21、60和100天后进行评价。球孢白僵菌减少了害虫种群数量,但与对照差异不显著,而金龟子白僵菌在不同处理间对受损茎的影响差异显著。绿僵菌在田间对绿僵菌的幼虫和成虫均有较好的控制效果,且白粉剂制剂的作用速度快于白粉剂制剂,225 g分生菌/ 1的剂量足以减少绿僵菌的数量。
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