In vivo therapeutic effect of wollastonite and hydroxyapatite on bone defect

IF 3.9 3区 医学 Q2 ENGINEERING, BIOMEDICAL Biomedical materials Pub Date : 2019-10-08 DOI:10.1088/1748-605X/ab4238
Rui Ge, Cong Xun, Jingzhou Yang, Weitao Jia, Yuancheng Li
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引用次数: 17

Abstract

The treatment of large-area bone defects is a huge challenge and the current research hot spot is to prepare composite materials to promote the new bone formation. In this study, the rat skull defect was repaired by implanting pure wollastonite and hydroxyapatite composites, which proved that it has a good effect on the treatment of bone defects. 60 SD rats were used as research objects. The animals were randomly divided into wollastonite group, wollastonite-hydroxyapatite composite group and hydroxyapatite group. The three groups of bone scaffolds were filled into the rats’ skull defects. At 6 and 12 weeks after surgery, we conducted Micro-CT analysis, HE staining, Masson trichrome staining, Alizarin red staining and Microfil analysis, to assess the therapeutic and regeneration effects of three groups. At 6 weeks after implantation, the morphology results showed that little newly formed bone was observed in wollastonite group, on the contrary, more new bone in the surgical defects formed in the wollastonite-hydroxyapatite composite group and hydroxyapatite group. At 12 weeks after surgery, histology analyses revealed that the regenerated bone became more mature in each groups. The morphology showed that the maturity of new bone was improved and the scaffold material was partially absorbed in wollastonite-hydroxyapatite composite group. CT scan observation showed that on the coronal plane, the defect repair area of wollastonite-hydroxyapatite composite group was integrated with the surrounding normal bone tissue, and the sacffold material was tightly integrated with the defect edge. The results of Microfil showed that compared with wollastonite group and hydroxyapatite group, wollastonite-hydroxyapatite composite group formed more blood vessels after 12 weeks of surgery. The wollastonite-hydroxyapatite composite biomaterial can promote the formation and growth of new bone in the defect area, and it is considered safe.
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硅灰石和羟基磷灰石对骨缺损的体内治疗作用
大面积骨缺损的治疗是一个巨大的挑战,目前的研究热点是制备复合材料促进新骨的形成。本研究通过植入纯硅灰石和羟基磷灰石复合材料修复大鼠颅骨缺损,证明其对骨缺损的治疗效果良好。以60只SD大鼠为研究对象。随机分为硅灰石组、硅灰石-羟基磷灰石复合组和羟基磷灰石组。三组骨支架均填充于大鼠颅骨缺损处。术后6周、12周进行Micro-CT分析、HE染色、Masson三色染色、茜素红染色、Microfil分析,评价三组治疗及再生效果。植入后6周,形态学结果显示硅灰石组新生骨很少,而硅灰石-羟基磷灰石复合组和羟基磷灰石组的手术缺损新生骨较多。术后12周,组织学分析显示各组再生骨更加成熟。形态学观察表明,硅灰石-羟基磷灰石复合材料组新骨的成熟度提高,支架材料被部分吸收。CT扫描观察显示,冠状面硅灰石-羟基磷灰石复合组缺损修复区与周围正常骨组织融合,支架材料与缺损边缘紧密结合。Microfil结果显示,与硅灰石组和羟基磷灰石组相比,硅灰石-羟基磷灰石复合组在手术12周后血管形成更多。硅灰石-羟基磷灰石复合生物材料可以促进缺损区新骨的形成和生长,被认为是安全的。
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来源期刊
Biomedical materials
Biomedical materials 工程技术-材料科学:生物材料
CiteScore
6.70
自引率
7.50%
发文量
294
审稿时长
3 months
期刊介绍: The goal of the journal is to publish original research findings and critical reviews that contribute to our knowledge about the composition, properties, and performance of materials for all applications relevant to human healthcare. Typical areas of interest include (but are not limited to): -Synthesis/characterization of biomedical materials- Nature-inspired synthesis/biomineralization of biomedical materials- In vitro/in vivo performance of biomedical materials- Biofabrication technologies/applications: 3D bioprinting, bioink development, bioassembly & biopatterning- Microfluidic systems (including disease models): fabrication, testing & translational applications- Tissue engineering/regenerative medicine- Interaction of molecules/cells with materials- Effects of biomaterials on stem cell behaviour- Growth factors/genes/cells incorporated into biomedical materials- Biophysical cues/biocompatibility pathways in biomedical materials performance- Clinical applications of biomedical materials for cell therapies in disease (cancer etc)- Nanomedicine, nanotoxicology and nanopathology- Pharmacokinetic considerations in drug delivery systems- Risks of contrast media in imaging systems- Biosafety aspects of gene delivery agents- Preclinical and clinical performance of implantable biomedical materials- Translational and regulatory matters
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