Toxicity and Anti-Oxidation Capacity of The Extracts from Caulerpa lentillifera

Q3 Health Professions Chiang Mai University journal of natural sciences Pub Date : 2021-06-16 DOI:10.12982/cmujns.2021.065

Supawadee Osotprasit, Tepparit Samrit, Athit Chaiwichien, N. Changklungmoa, Krai Meemon, Nakorn Niamnont, Preeyanuch Manohong, Kunwadee Noonong, M. Tamtin, P. Sobhon, P. Kueakhai

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引用次数: 2

Abstract

Caulerpa lentillifera (sea grape) has been widely used in pharmaceutical industry and health-care products in Thailand. In this study, we attempted to evaluate the toxicity and antioxidant capacity of sea grape extracts in five fractions (ethanol- CLET, hexane- CLHE, ethyl acetate- CLEA, butanol-CLBU, and aqueous-CLAQ). The extracts were evaluated for cytotoxicity by MTT and LDH assays on four cell lines, fibroblast (L929), macrophages (RAW 264.7), hepatocytes (FL83B), and keratinocytes (HaCaT). Genotoxicity was tested by comet assay and micronucleus assay on human lymphoblast cells (TK6). The antioxidant capacity was measured by DPPH and ABTS scavenging assays. Our results demonstrated low cytotoxicity and genotoxicity of CLET, CLBU and CLAQ. When tested by DPPH and ABTS assays, CLET, CLEA, and CLHE showed high antioxidant activity. In conclusion, CLET, CLBU, and CLAQ demonstrated no toxic effects, and CLET, CLEA, and CLHE exhibited high antioxidant capacity. Therefore, our results indicated that CLET, CLEA, and CLHE could be consumed safely at doses lower than 500 and 200 μg/ml for CLHE and CLEA, respectively. Keywords: Anti-oxidation, Caulerpa lentillifera, Cytotoxicity, Genotoxicity

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小扁豆提取物的毒性及抗氧化能力研究

海葡萄在泰国的医药工业和保健品中有着广泛的应用。在本研究中，我们试图评估海葡萄提取物的五种组分（乙醇-CLET、己烷-CHE、乙酸乙酯-CLEA、丁醇-CLBU和水性CLAQ）的毒性和抗氧化能力。通过MTT和LDH测定对四种细胞系（成纤维细胞（L929）、巨噬细胞（RAW 264.7）、肝细胞（FL83B）和角质形成细胞（HaCaT））的细胞毒性评估提取物。用彗星试验和微核试验对人成淋巴细胞（TK6）进行遗传毒性试验。通过DPPH和ABTS清除试验测定抗氧化能力。我们的结果表明CLET、CLBU和CLAQ具有较低的细胞毒性和遗传毒性。当通过DPPH和ABTS测定测试时，CLET、CLEA和CLHE显示出高抗氧化活性。总之，CLET、CLBU和CLAQ没有表现出毒性作用，并且CLET、CLEA和CLHE表现出高抗氧化能力。因此，我们的研究结果表明，CLET、CLEA和CLHE在剂量分别低于500和200μg/ml时可以安全食用。关键词：抗氧化；扁豆苔；细胞毒性；基因毒性

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Chiang Mai University journal of natural sciences Health Professions-Health Professions (miscellaneous)

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1.70

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