The effects of desiccation and salinity gradients in the PSII photochemical efficiency of a subtidal brown alga, Saccharina japonica (Laminariales) from Hokkaido, Japan

Abstract

We determined the response of photochemical efficiency to desiccation and salinity in an edible brown alga, Saccharina japonica (var. japonica), using a pulse amplitude modulation chlorophyll fluorometer. Natural populations of this species in Muroran, Hokkaido, Japan are most of the time immersed in the subtidal zone; however, those at the uppermost part of the sublittoral are sometimes emersed during low tide. In the laboratory experiment, the alga under aerial exposure up to 8 h under dim light at 20°C and 50% humidity showed that the effective quantum yields (ΔF/Fm′) of Photosystem II quickly declined after more than 45 min of emersion; ΔF/Fm′ also failed to recover to the initial level even after 1 day of rehydration in seawater. Under emersion, the ΔF/Fm′ values were relatively stable and above 0.5 when the absolute water content (AWC, %) was greater than 50%; however, it declined as the AWC decreased. Once the algae were dehydrated to an AWC of less than 50%, their ΔF/Fm′ did not return to the initial level despite subsequent reimmersion in seawater, suggesting the low ability of photosynthetic recovery from dehydration. Results of in situ measurements in Muroran showed that the ΔF/Fm′ of S. japonica declined during the tidal exposure; however, it recovered after they were again immersed in seawater due to the rising tide. Furthermore, S. japonica showed a stenohaline photosynthetic response between 20 and 40 psu, as their ΔF/Fm′ values were found to be unchanged in these salinities after 5 days of laboratory culture; this suggests the seaweed’s photosynthetic tolerance and/or acclimation within such a relatively narrow range, which might be one of the traits that preclude its occurrence in the intertidal and brackish waters. Indeed, desiccation risk and fluctuating salinity are important limiting factors that influence the stability of kelp beds for sustainable utilization in the regional fishery including mariculture of this species.