Determination of Vitamin E Levels in Walnuts (Juglans regia L.) Grown in the Lake Van Basin by HPLC

Abstract

Aim of study: The present study describes determination of vitamin E levels in Walnuts (Juglans regia L.) Grown in the Lake Van basin. Area of study: Adilcevaz, Bahçesaray, Çatak, Edremit, Gevaş regions in the Lake Van basin and in the center of Van. Material and methods: Walnuts were collected regions and stored in a deep freezer for analysis. On the day of analysis, 10 pieces of fresh and dried walnut samples belonging to each region were taken and broken, and a homogeneous mixture was obtained by pulverizing them and determined by HPLC. Main results: The amounts of α-Tocopherol of the samples in fresh walnuts were found to be 2.36 ± 0.01 μg/g, 5.01 ± 0.31 μg/g, 6.23 ± 0.15 μg/g, 7.20 ± 0.28 μg/g, 3.99 ± 0.46 μg/g, 5.05 ± 0.30 μg/g, respectively, and the amounts of γ-Tocopherol were found to be 23.80 ± 0.52 μg/g, 16.54 ± 1.08 μg/g, 14.82 ± 0.46 μg/g, 17.34 ± 0.78 μg/g, 11.91 ± 0.40 μg/g, 15.57 ± 0.90 μg/g, respectively, according to the regions. The amounts of α-Tocopherol in dried walnuts were found to be 5.13 ± 0.26 μg/g, 6.84 ± 0.31 μg/g, 7.58 ± 0.40 μg/g, 10.49 ± 0.51 μg/g, 8.65 ± 0.34 μg/g, 5.82 ± 0.19 μg/g, respectively, and the amounts of γ-Tocopherol were found to be 33.10 ± 1.65 μg/g, 21.97 ± 0.87 μg/g, 20.98 ± 0.83 μg/g, 29.19 ± 0.80 μg/g, 21.09 ± 0.24 μg/g, 18.41 ± 0.82 μg/g, respectively, according to the regions. Highlights: The results obtained in the study are considered, it is observed that the walnuts grown in Adilcevaz, Bahçesaray, Çatak, Edremit, Gevaş and Van-Center regions are of high quality.