Immobilization of Pseudomonas aeruginosa FNCC-0063 on Calcium Alginate and Its Application as Bioabsorbent

Iin Novianty, A. Suratman, Mudasir
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Abstract

Biosorption of Cu (II) using bacteria Pseudomonas aeruginosa FNCC-0063 was immobilized on calcium alginate (PAI). This research examined the effect of various parameters such as pH, contact time, and initial Cu (II) concentration. The biosorption mechanism of Cu (II) was studied by sequential desorption with H2O, KNO3 1 M, HNO3 0.5 M and Na2EDTA 0.1 M. Cu (II) concentration was analyzed using atomic absorption spectrophotometer (AAS). The results showed that optimum Cu (II) ion biosorption occurred at a pH biosorption rate constant of 0.03724 g mg-1.min-1. The kinetics studies showed that Cu (II) biosorption follows pseudo-second-order. The biosorption capacities of 36.60 mg/g. Cu (II) Biosorption followed the Freundlich equation, as shown by a high correlation coefficient (R2) of about 0.99. Ionic bonds dominated the biosorption mechanism of Cu (II) ion on immobilized PAI.
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铜绿假单胞菌fnc -0063在海藻酸钙上的固定化及其生物吸附剂的应用
采用海藻酸钙(PAI)固定铜绿假单胞菌fnc -0063对Cu (II)的生物吸附。本研究考察了pH、接触时间和初始Cu (II)浓度等不同参数的影响。采用水、KNO3 1 M、HNO3 0.5 M、Na2EDTA 0.1 M的顺序解吸法研究了Cu (II)的生物吸附机理。结果表明,pH吸附速率常数为0.03724 g mg-1 min-1时,Cu (II)离子吸附效果最佳。动力学研究表明,Cu (II)的生物吸附服从准二阶。生物吸附量为36.60 mg/g。Cu (II)的生物吸附符合Freundlich方程,相关系数(R2)约为0.99。Cu (II)离子在固定化PAI上的生物吸附机制以离子键为主。
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